【作者】 王丹凤；

【导师】 郑沙盟；

【作者基本信息】 浙江中医药大学 ， 中医五官科学， 2011， 硕士

【摘要】 目的观察自拟脱敏止嚏汤对变应性鼻炎(AR)大鼠模型鼻粘膜中IL-18和EotaxinmRNA表达水平的影响,探讨其治疗变应性鼻炎的作用机理,为该方在临床治疗AR提供理论依据。方法将40只健康SD大鼠随机分成正常对照组、过敏性鼻炎模型组、地塞米松(DXM)治疗组和自拟脱敏止嚏汤治疗组,每组10只,采用卵清蛋白(OVA)为致敏原制备AR大鼠模型,治疗组在腹腔注射全身致敏结束后分别给予DXM、中药汤剂干预14天并给予OVA滴鼻维持过敏状态,而正常对照组则用生理盐水代替OVA及干预药物。用被动皮肤过敏原试验(PCA)检验大鼠模型制备及药物干预后情况,用ELISA法检测血清中IL-18和Eotaxin的含量,用RT-PCR法检测各组大鼠鼻粘膜中IL-18和EotaxinmRNA表达水平。结果1.大鼠鼻部症状计分：治疗前,模型组计分高于正常组(P<0.05),治疗后,中、西药治疗组计分低于模型组(P<0.01),差异有显著性,中、西药治疗组间差异无统计学意义(P>0.05)。2.血清IL-18的含量及鼻粘膜中IL-18mRNA的表达水平：治疗前,模型组血清中IL-18的含量和鼻粘膜中IL-18mRNA的表达水平均低于正常组(P<0.01),治疗后,中、西药治疗组血清中IL-18的含量和鼻粘膜中IL-18mRNA的表达均高于模型组(P<0.01),差异有显著性,两治疗组间差异无统计学意义(P>0.05)。3.血清Eotaxin的含量及鼻粘膜中EotaxinmRNA表达水平：治疗前,模型组血清中Eotaxin的含量和鼻粘膜中EotaxinmRNA表达水平均高于正常组(P<0.01),治疗后,中、西药治疗组血清中Eotaxin的含量和鼻粘膜中EotaxinmRNA表达水平均低于模型组(P<0.01),差异有显著性,中、西药治疗组间差异无统计学意义(P>0.05)结论自拟脱敏止嚏汤能明显改善变应性鼻炎大鼠的鼻痒、喷嚏、清涕等症状,通过下调变应性鼻炎大鼻黏膜EotaxinmRNA的表达,上调IL-18mRNA的表达,调节Thl和Th2的偏移,纠正失衡的Th细胞因子网络系统,达到治疗AR的目的。主题词：自拟脱敏止嚏汤,变应性鼻炎,白介素18,嗜酸性粒细胞趋化因子更多还原

【Abstract】 Objective To observe the effect of ZiNiTuoMinZhiTi-decoction to allergic rhinitis on the level of gene expression of IL-18mRNA and EotaxinmRNA in nasal mucosa in rat model of allgegic rhinitis,and to show the part action mechanism of the ZiNiTuoMinZhiTi-decoction treating allergic rhinitis,then explore the most effective way to treat allergic rhinitis.We are going to establish the theory basis of clinic treatment of AR through this research.Methods Forty rats were equally randomized into 4 groups:normal control group, allergic rhinitis(AR) group and AR+DXM group and AR+ ZiNiTuoMinZhiTi-decoction group with 10 cases in each group. Establish allergic rhinitis model of rats by injecting vaccine (OVA+Alumina), Rats in AR+ DXM group were treated with DXM for 2 weeks after OVA challenge and Rats in AR+ ZiNiTuoMinZhiTi-decoction group were treated with the ZiNiTuoMinZhiTi-decoction for 2 weeks after OVA challenge and rats in normal control group received normal saline. Passive cutaneous anaphylaxis(PCA) test was performed in all rats and the content IL-18 and Eotaxin in the serum were determined by Elisa and the level of gene expression of IL-18 and Eotaxin were determined by RT-PCR method.Results 1.Observation of nasal symptoms on the AR rats before and after treatment and marks showed that,before treatment,the nasal symptoms of normal group had significant difference with other three groups,after treatment,symptoms of western medicine group had no obvious difference compared with that of TCM group(P>0.05),while both had lower marks than mode group (P<0.01).2. Serum IL-18 content and The level of gene expression of IL-18:before treatment,model group IL-18 content and the level of gene expression of IL-18 are below normal group (p<0.01),after treatment,group,western medicine therapy in serum IL-18 content and the level of gene expression of IL-18 higher than in model group(p<0.01),there is a significant difference between the two treatment groups,sex difference was satistically significant(p>0.05).3. Serum Eotaxin content and nasal mucosa EotaxinmRNA in expression level:before treatment,model group Eotaxin content in serum and nasal mucosa EotaxinmRNA expression level are higher in normal group(p<0.01),after treatment,in treatment group,the western Eotaxin content in serum in and nasal mucosa EotaxinmRNA expression level are below model group(p<0.01),group,medium,westem medicine therapy not statistically significant between-group difference(p>0.05).Conclusion ZiNiTuoMinZhiTi-decoction has good effect on allergic rhinitis nasal deschange,sneezing,clear cut Ti wait for a symptom,through large allergic rhinitis nasal mucosa, up-regulated IL-18mRNA expression level and down-regulated EotaxinmRNA expression level in the nasal septum mucosa of allergic rhinitis rat and adjust the offset,correcting unbalanced Th cytokine network system,to achieve the purpose of treatment of the AR.更多还原