【作者】 刘雪润；

【导师】 杨世林； 许琼明；

【作者基本信息】 苏州大学 ， 药物化学， 2011， 硕士

【摘要】 自1998年我们首次报道多氧取代环己烯类化合物zeylenone及其抗肿瘤活性后,一直坚持研究该类成分。为了寻找新型抗肿瘤活性化合物,利用有关植物资源,本论文对该属植物紫玉盘(Uvaria microcarpa Champ. ex Benth)的叶进行了系统的化学成分研究。用70%乙醇回流提取紫玉盘叶三次,所得提取液浓缩后分别用石油醚、氯仿、乙酸乙酯、正丁醇萃取,得到四个部位。利用反复硅胶柱层析、Sephadex LH-20凝胶柱层析和制备HPLC等手段对这四分部位进行分离,得到25个化学成分,通过NMR、MS、IR、UV等光谱分析和理化常数,鉴定了22个化合物,分别为:硬脂酸(ZL-1)、乌苏酸(ZL-2)、β-谷甾醇(ZL-3)、蒲公英赛醇(ZL-4)、苯甲酸(ZL-5)、对羟基苯甲酸(ZL-6)、齐墩果酸(ZL-7)、咖啡酸(ZL-8)、阿魏酸(ZL-9)、3-甲氧基-4-羟基-苯甲酸(ZL-10)、Microcarpol A (ZL-11)、Microcarpol B (ZL-12)、延胡索乙素(ZL-13)、(E)-1-(2,4-Dihydroxy-3,6-dimethoxyphenyl)-3-phenylprop-2-en-1 -one (ZL-14)、Zeylenol (ZL-15)、Ellipeiopsol B (ZL-16)、β-胡萝卜苷(ZL-17)、马兜铃内酰胺AⅡ(ZL-18)、槲皮素(ZL-19)、山奈酚(ZL-20)、山奈酚-3-O-β-D-芸香糖苷(ZL-21)、芦丁(ZL-22)。其中,ZL-11、ZL-12为新化合物;ZL-15、ZL-16为多氧取代环己烯类化合物; ZL-7、ZL-8、ZL-10、ZL-14为首次从该属植物中分离得到的化合物。此外,本文综述了紫玉盘属植物的化学成分和药理研究概况。更多还原

【Abstract】 Our research group reported a new polyoxygenated cyclohexene-zeylenone and its antitumour activities for the first time in 1998, since then, we persisted in study on polyoxygenated cyclohexenes for several years. In order to research the resourse of Uvaria species and to discover more antitumor agents from nature, Uvaria microcarpa Champ. ex Benth growing in Guangxi province was subjected to phytochemical studies. The leaves were extracted with 70% EtOH three times under reflux. The EtOH extract was partitioned with petroleum ether, chloroform, ethyl acetate and n-butanol. Twenty-five compounds were isolated using silical gel, Sephedax LH-20 and semi-HPLC colomn chromatography and twenty-two were structurally identified by means of various modern spectroscopic techniques (NMR, MS, IR,UV and so on ) and their physical properties.They are as follows: stearic acid (ZL-1)、ursolicacid (ZL-2)、β-sitosterol (ZL-3)、taraxerol (ZL-4)、benzoic Acid (ZL-5)、hydroxybenzoic acid (ZL-6)、oleanolic Acid (ZL-7)、caffeic acid (ZL-8)、ferulic acid (ZL-9)、vanillic acid (ZL-10)、Microcarpol A (ZL-11)、Microcarpol B (ZL-12)、tetrahydropalmatie (ZL-13) (E)-1-(2,4-Dihydroxy-3,6-dimethoxyphenyl)-3-phenylprop-2-en-1-one (ZL-14)、Zeylenol (ZL-15)、Ellipeiopsol B (ZL-16)、daucosterol (ZL-17)、Aristololactam AⅡ(ZL-18)、quercetin (ZL-19)、kaempferol (ZL-20)、kaempferol-3-O-β-D-rutinoside (ZL-21)、rutin (ZL-22)。Among them, ZL-11 and ZL-12 were new compounds; ZL-15 and ZL-16 were polyoxygenated cyclohexenes compounds; ZL-7、ZL-8、ZL-10 and ZL-14 were isolated in Uvaria genus for the first time.This thesis also reviewed advances of the chemical and pharmacological studies of Uvaria genus.更多还原