节点文献
单糖检测体系的构建及性能测试研究
Construction and Performance Research of the Sensing Systems for Monosacchrides
【作者】 王国峰；
【作者基本信息】 山西大学 ， 有机化学， 2011， 硕士
【摘要】 分子识别一直是有机化学中最具有吸引力的领域之一,从对离子到简单分子以及生物复杂分子的识别整个过程都得到了科学研究者的广泛关注。从超分子识别角度来看,所谓分子识别就是主体(或受体)对客体(或底物)选择性结合并产生某种特定功能的过程。随着科学技术的快速发腱,对简单离子的识别已经无法满足社会发展的要求,对分子识别尤其是具有生物功能的分子如糖、氨基酸等识别是目前化学家、生物学家及医学家研究的热点。本学位论文选取在生命体中具有重要生物功能和代谢功能的糖分子进行识别研究。目的是得到高选择性的单糖识别体系,为研究更复杂的生命过程及临床医学糖检测提供可靠的依据和途径。另外,学位论文设计并合成出一系列在医药领域方面有重要作用的吲哚衍生物药物中间体。具体包括以下五个章节。第一章,对单糖分子识别传感器做简要的综述,提出本论文研究思路。第二章,设计并合成苯一苯水溶性聚合物PPP-SO3-Na+和三种硼酸取代viologens猝灭接受体,并通过组合得到不同组合体系。在接近生理条件下,利用荧光光谱对组合体系和单糖的相互作用进行了研究。研究结果为：o-BBV/PPP-SO3-Na+、m-BBV/PPP-SO3-Na+及p-BBV/PPP-SO3-Na+三种组合体系对D-果糖都具有高的选择性和灵敏性,对果糖的检测范围为1.0mM-200.0mM。三种组合体系中发光体和猝灭结合体的最佳组合比例分别为：o-BBV/PPP-SO3-Na+=3/1、m-BBV/PPP-SO3-Na+= 1/1及p-BB V/PPP-SO3-Na+=3/1(mol)。第三章,设计并合成苯-芴水溶性聚合物FPP-SO3-Na+,并分别与三种硼酸取代viologens猝灭接受体组合识别体系,利用荧光光谱对组合体系和单糖的相互作用进行了研究。研究结果为：o-BBV/FPP-SO3-a+和m-BBV/FPP-SO3-a+组合体系也都对D-果糖都具有高的选择性和灵敏性。虽然p-BBV/FPP-SO3-Na+组合体系对果糖也具有选择性,但是识别响应信号很弱。第四章,设计并合成一系列在医药及染料领域有重要作用的吲哚衍生物。第五章,研究工作总结与展望。
【Abstract】 Molecular recognition is one of the most attractive fields in organic chemistry. The recognition processes from the ions to simple molecules and complex biological molecular have attracted widely attention by chemists and biologists et al. With the development of modern science, the recognition of ion has not meeted the demand of social development. Molecular sensing of biological analytes with a metabolic function in living organisms has attracted extensive attention and well-designed recognition systems are crucial for high sensitivities.Saccharide molecules which have important function in organism were studied as our research object of the dissertation. The purpose is to get highly selectivity recognition system for monosaccharide and provides dependable basis for more complex life process and logical approach to the identification of clinical medical saccharide. Addtionally, we synthesised several indole derivatives which had important use in medicine and dye fields. This paper mainly includes five chapters as follows.Chapter 1:Briefly introduction of saccharide sensor and bring forward the topic of this paper.Chapter 2:Design and synthesis benzene-benzene water solubility organic polymer PPP-SO3-Na+ and three kinds of boric acid-substitude viologens quencher. The sensing actions of different sensing system to monosaccharides have been studied by fluorescence emission spectrum at physiological conditions. The results showed that the three combination systems (o-BBV/PPP-SO3-Na+、m-BBV/PPP-SO3-Na+ and p-BBV/PPP-SO3-Na+) had high selectivity and sensitivity for D-fructose. The detection range for D-fructose was from 1.0 mM to 200.0mM. The optimal ratios for fluorophore and quencher of the three system were o-BV/PPP-SO3-Na+ 3/1、m-BBV/PPP-SO3-Na+=1/1 andp-BBV/PPP-SO3-Na+=3/1, respectively.Chapter 3:Design and synthesis benzene-fluorene water solubility organic polymer FPP-SO3-Na+. The sensing actions of different sensing system to monosaccharides have been studied by fluorescence emission spectrum at physiological conditions. The results showed that the combination of o-BB V/FPP-SO3-Na+ and m-BBV/FPP-SO3-Na+ had higher selectivity for D-fructose. Additionally, although the system p-BBV/PPP-SO3-Na+ had selectivity for D-fructose, the signal of the recognition responsewas very weak.Chapter 4:Synthesis several indole derivatives.Chapter 5:Summary and prospects.