【作者】 贺鹏飞；

【导师】 黄登宇； 彭晓光；

【作者基本信息】 山西大学 ， 生物化学与分子生物学， 2011， 硕士

【摘要】 山西老陈醋以其“酸醇厚、味清香、回味绵、质浓稠、色棕红和久贮不变质”等特点著称于世。酿醋业作为我省的名片产品,极大的推动了我省的经济发展,但是繁荣的背后也带来了不少的隐患,使得目前老陈醋市场较乱。本文旨在寻求一种有效的办法探索老陈醋中理化指标与特征性成分川芎嗪之间的内在关系。首先,通过对山西老陈醋中10个性能比较稳定的醋样进行常规成分的测定,得出不同老陈醋品种中以D06的酸度和各有效常规成分含量最高,同时D06中又以3年,5年陈酿常规有效成分最稳定,营养成分最高。其次,在不吸附样品前提下,选用C 18固相萃取小柱对山西老陈醋样品进行预处理,对老陈醋中的6种主要有机酸组分进行了定量测定。最后,通过HPLC得出醋样中川芎嗪的含量,利用SPSS对相关的常规成分进行线性回归分析,经过验证发现,由pH和总酸理化指标组成的方程与预期值具有较好的拟合度。利用灰关联对老陈醋中有机酸和川芎嗪进行评估,得出X6的综合指标表现最优越,与参考品种最接近且川芎嗪含量最大,可以作为最优品种参考,同时发现柠檬酸的理化指标与川芎嗪含量的关联度最大,进而可以以柠檬酸的含量水平推断出川芎嗪的含量高低。计划下一步继续寻求理化指标与川芎嗪之间的联系,希望能够通过它们之间的内在联系找出川芎嗪含量的范围,使之能用于山西老陈醋的食品检验。更多还原

【Abstract】 Shanxi Overmature Vinegar with its acid mellow, fragrant flavor, aftertaste cotton, quality thick, reddish brown color and a long storage without deterioration, and so famous for. Vinegar products industry as a business card in our province, greatly promoted the economic development of our province, but the prosperity has also brought back a lot of hidden dangers, making the market more mature vinegar current chaos. This paper aims to find a way to explore the old vinegar and effective physical and chemical indicators and characteristic components of the intrinsic relationship between TMP.First of all, by Shanxi Overmature Vinegar,10 personality will be more stable component in vinegar-like determination, come to different varieties to mature vinegar D06 acidity and the content of effective component in the highest, while D06 turned around 3 years,5 Aging active ingredient in the most stable routine, the highest nutrient content.Secondly, the premise does not adsorb the sample, the choice of C 18 solid-phase extraction column to the Shanxi Vinegar sample pretreatment, the old vinegar in the 6 major components of the quantitative determination of organic acids.Finally, obtained by HPLC content of TMP vinegar samples, using SPSS general composition of the relevant linear regression analysis, after finding that the physical and chemical indicators of pH and total acid composition of the equation and the expected value of the degree of good fit. Using Grey Relational Vinegar TMP assessment of organic acids and obtained the performance of the most comprehensive index X6 superior varieties with reference to the closest and most TMP content, can be used as reference for the best varieties, while the citric acid found in index Associated with the greatest degree of TMP content, then the level of citric acid can infer the content of the level of TMP..The next step to continue to seek the links between physical and chemical indicators and TMP, hoping through them to find out the intrinsic link between the scope of TMP content, so that it can be used Shanxi Overmature vinegar’s inspection.更多还原