【作者】 杨洪权；

【导师】 陈建新；

【作者基本信息】 福建师范大学 ， 医学光子技术与仪器， 2011， 硕士

【摘要】 许多癌症发生于层状结构的生物组织,且大多数胃癌出现在胃粘膜。由于诊断效果欠佳,胃癌是第二大常见的致死性癌症。基于双光子激发荧光和二次谐波产生的多光子激发显微技术是一种新兴的非线性光谱成像技术,它结合了双光子激发显微术和光谱测量技术,因此它能同时获得生物组织不同深度的图像和光谱信息。在本论文中,首先,通过结合双光子激发荧光成像和二次谐波产生成像,我们观察了人体胃粘膜的固有层。研究表明,用多光子激发显微技术能够有效地描述人体正常的胃粘膜的固有层的微结构。接着,利用多光子激发显微技术,我们研究了人体正常的和癌变的胃组织的粘膜层和粘膜下层,并建立了区分这两层的诊断特征。研究结果显示,多光子激发显微技术不仅能表现正常的和癌变的胃组织的粘膜层和粘膜下层的微结构,还能测量这两层中异常细胞的分布和含量。更重要的是,这些结果为建立胃癌的临床诊断标准奠定了实验基础。总之,我们的研究表明,多光子激发显微技术极有可能为人体胃组织的生理分析和病理诊断提供更精确、更丰富的信息。随着临床用微型化的多光子激发显微术和多光子探针技术的发展,我们的实验研究结果将有助于利用多光子内窥镜技术实现胃癌的早期、活体和实时的诊断。更多还原

【Abstract】 Many neoplasms occur in biological tissues with a fine stratified structure, and most of stomach cancers develop in the gastric mucosa. Due to poor diagnosis, the stomach cancer is the second most common cause of death. Multiphoton excitation microscopy (MPM) is an emerging nonlinear spectral imaging based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). This microscopy combines two-photon excitation microscopy with spectral measurement technique, so it can be simultaneously used for obtaining tissue image and spectra at different depth. In this thesis, firstly, combining TPEF and SHG, the lamina propria of normal human gastric mucosa was observed. Our results demonstrated that MPM can be very effective to characterize the microstructure of lamina propria in normal human gastric mucosa. Then, by using MPM, the mucosa and submucosa of human normal and cancerous gastric tissues were investigated, and the diagnostic features for identifying the two layers were established. Our findings show that MPM can be capable of exhibiting the mucosal and submucosal microstructures of normal and cancerous gastric tissues, as well as the distribution and content of abnormal cells in the two layers. More importantly, the results can provide the groundwork for establishing clinical diagnostic criteria of gastric cancer. In a word, our findings suggested that MPM has great potential for providing more accurate and comprehensive information in physiological analysis and pathological diagnosis of human gastric tissue. With the development of clinically miniaturized MPM and multiphoton probe, our results can be helpful for applying multiphoton endoscopy to perform real-time in vivo early diagnosis of gastric cancer.更多还原