【作者】 张伟；

【导师】 林健敏；

【作者基本信息】 桂林医学院 ， 外科学， 2011， 硕士

【摘要】 目的:探讨DCR3对HCC的诊断价值和临床意义。方法:采用ELISA(enzyme linked immunosorbent assay)检测45例正常人血清中的DCR3(decoy receptor 3)水平;再对45例经手术后病理学证实的HCC(hepatocellularcarcinoma)患者分别用ELISA检测其血清中的DCR3水平,用IHC(immunohistochemistry)检测HCC癌组织中及癌旁组织中DCR3蛋白的表达;用RT-PCR(Reverse Transcription Polymerase Chain Reaction)检测其肿瘤组织中及癌旁组织中DCR3基因mRNA的表达。由SPSS13.0统计软件分析。计数资料比较用χ2检验,各组间的计量资料比较采用方差分析,方差不齐时作秩和检验。计算Pearson相关系数判断DCR3与其它变量的相关关系。影响DCR3的多因素分析采用多元线性逐步回归分析。结果:HCC组血清DCR3水平明显高于正常对照组(P<0.01),且与伴有肝硬化有关(P<0.05);DCR3蛋白在HCC癌组织中表达与血清水平呈正相关(r=0.395,P<0.01),但DCR3血清阳性率高于癌组织IHC(P<0.05),且DCR3蛋白在HCC癌旁组织中不表达;DCR3基因mRNA的表达与DCR3蛋白的表达基本相一致,相应癌旁组织中均为阴性。但其阳性率71.1%(32/45)介于血清学水平75.6%(34/45)和组织学水平60%(26/45)之间。结论:DcR3升高在HCC的发生发展及浸润转移中起重要作用,通过监测高危人群以及肝癌患者尤其是血清中DcR3水平可能对HCC的筛查、诊断和判断预后有一定意义。更多还原

【Abstract】 Objective: To investigate the diagnostic value andclinical significance of elevateddecoy receptor 3(DcR3)in patients with hepatocellularcarcinoma(HCC).Methods: Serum DcR3 levels were measured using enzyme-linked immunosorbent assay(ELISA) in 45 patients with HCC and in the healthy individuals.The expression of DcR3 in HCC tissue was evaluated by immunohistochemistry(IHC).Reverse Transcription Polymerase Chain Reaction(RT-PCR) is used to evaluateThe expression of mRNA about DcR3Results: The levels of serum DcR3 in HCC patients were higher than that in healthy individuals (P<0.01).The level of serum DcR3 in HCC patients was correlated with the development of cirrhosis(P<0.05).There was a positive correlation betweenthe levels of DcR3 in the serum and HCC tissue(r=0.395,P<0.01). The positive rate of DcR3 inthe serum was significantly higher than that in HCC tissue(P<0.05). DcR3 is negative in the neighboring tissue around The sensitivities of single. The expression of mRNA about DCR3 and DCR3 protein expression basically consistent, is negative in the corresponding neighborly tissue around HCC. But its positive 71.1 percent (32/45) is between serological level 75.6% (34/45) and histological level 60 percent (26 / between 45). Conclusions: Elevated DcR3 may play an important role in the pathogenesis,development,invasion and metastasis of HCC.It may be of great significance for the screening,diagnosis and prognosis of HCC to simultaneously monitor the levels of DcR3 expecially in the serum level of high-risk populations and HCC patients.更多还原