【作者】 肖学敏；

【导师】 涂亚庭；

【作者基本信息】 华中科技大学 ， 皮肤性病学， 2011， 硕士

【摘要】 目的:研究IGFBP7在人皮肤鳞状细胞癌(简称鳞癌)组织及细胞中的表达情况探讨IGFBP7与上皮细胞间质转型(epithelial-mesenchymal transition, EMT)的上皮标记蛋白细胞角蛋白(cytokeratin,CK)在鳞癌中的表达及两者之间的相关性。方法:采用免疫组织化学亲和素-生物素-过氧化物酶(Avidin-Biotin-Peroxidase Complex,SABC)法检测50例皮肤鳞状细胞癌皮损和14例正常对照皮肤组织中IGFBP7表达;同时用实时荧光定量PCR(Real time-PCR)和免疫印迹(western-blot)方法检测人永生化角质形成细胞系HaCaT和皮肤鳞癌细胞系A431中IGFBP7各自的表达情况。结果: IGFBP7在正常皮肤组织中的阳性率为100%,而皮肤鳞状细胞癌组织中表达的阳性率为58.0%,两者表达有显著性差异(P<0.01);IGFBP7在高分化和低分化SCC中均低表达,但在低分化的SCC中的表达更少(P<0.01);IGFBP7和CK在皮肤鳞状细胞癌中的表达具有相关性(r=0.812,P<0.01);永生化角质形成细胞系HaCaT中IGFBP7的mRNA和蛋白表达水平均高于人皮肤鳞癌细胞系A431。结论: IGFBP7在人皮肤鳞癌组织及细胞中低表达,IGFBP7与肿瘤的病理学分级有关,且和CK表达呈正相关;IGFBP7的表达缺失可能在皮肤鳞状细胞癌的发生发展中起到重要作用,为皮肤鳞癌的诊断及治疗研究提供实验基础。更多还原

【Abstract】 Objectives : To study the expression of IGFBP7,a recently described member of insulin-like growth factor binding protein(IGFBP) family, in the cutaneous squamous cell carcinoma(SCC) and human SCC cell line and analyse the correlation between the expression of IGFBP7 and cytokeratin, which is considered to be a distinctive indicator of epithelial-mesenchymal transition (EMT).Methods : immunohistological staining method was applied to analyze the location of IGFBP7 expressions in 50 human squmaous cell carcinoma and 14 normal skin tissues.In addition, The expression of IGFBP7 messenger ribonucleic acid (mRNA) and protein was evaluated in the HaCaT cell line and A431 cell line by Real-time PCR and western-blot seperately.Results :The express rate of IGFBP7 is 58.0% in SCC specimens and 100% in the normol skin, There was significant differences between squamous cell carcinoma and normal skin tissues(P<0.01).The percentage of IGFBP7-positive cells in well - differentiated SCC specimens was also substantially higer than that in poorly -differentiated SCC specimens (P < 0.01). Furthermore, the expression of IGFBP7 was positively associated with the expression of cytokeratin (r=0.812,P<0.01). The lower mRNA and protein expression of IGFBP7 was seen in A431,shown by Real-time PCR and Western blotting(P<0.01).Conclusions: IGFBP7 is expressed constitutively and more intensely in normal tissues and cell line than that in SCC.The loss of IGFBP7 may play an important role in the pathogenesis and the development of skin squamous cell carcinoma .更多还原