【作者】 潘娟；

【导师】 张灵武；

【作者基本信息】 宁夏医科大学 ， 妇产科学， 2011， 硕士

【摘要】 目的研究杏仁核电刺激点燃的癫痫模型大鼠动情周期、卵巢形态学及生长卵泡的超微变化。方法取35只8周龄SD雌性大鼠,随机分为癫痫模型组(n=15)、假手术组(n=10)和对照组(n=10)。癫痫模型组,双极电极植入大鼠左侧杏仁基底外侧核,给予连续电刺激,建立电点燃的癫痫模型;假手术组,电极植入后不给予电刺激;对照组为正常雌性大鼠。所有大鼠每天清晨行阴道细胞涂片,以监测动情周期。癫痫模型大鼠在点燃20d后于动情周期的间期取双侧卵巢组织,同期其他两组大鼠也分别于动情间期摘取双侧卵巢组织,右侧卵巢做病理切片,HE染色后镜下阅片,采用Image-Pro Plus 6.0分析软件进行形态学分析,左侧卵巢选择窦状卵泡进行透射电镜观察。结果癫痫模型组大鼠失去正常动情周期,日均体重增加的克数及卵巢重量均较假手术组及对照组显著增高(P<0.05);病理显示,癫痫模型组大鼠卵巢切面面积增大,窦前卵泡、总生长卵泡数及闭锁卵泡显著增多(P<0.05),卵巢发生多囊性改变;电镜下显示癫痫组大鼠窦状卵泡中的颗粒细胞凋亡增多,增生的卵泡膜细胞更加富含脂滴。结论癫痫发作可影响大鼠排卵功能,并导致大鼠发生卵巢多囊性改变,生长卵泡凋亡增多,卵泡膜细胞增生分泌增强。更多还原

【Abstract】 To explore changes of estrous cycle and ovarian morphology and ultrastructure of growing follicles in amygdale-kindled seizures female rats.Methods Thirty five SD rats of 8-week-old were randomly divided to Kindling electrodes (n=15), sham-kindled (n=10), and controls (n=10). Kindling electrodes were implanted in the left basolateral amygdala in adult female rats. The rats were kindled by brief, daily, suprathreshold stimulations with a bipolar electrode. The sham-kindled rats only implanted bipolar electrode in the left basolateral amygdala. Controls were normal female rats. Estrous cycles were monitored with daily vaginal smears. Electrographic and behavioral seizures were monitored,.right Ovary tissues were taken after 20 days of kindled in the diestrus. At the same time, sham-kindled subjects and their yoked controls were sacrificed and their right ovaries were taken in their diestrus. Ovarian morphometric analyses were examined. Results Kindled rats lost ovarian periodicity and they displayed significantly weight gained and ovarian increasing(P<0.05). The result of HE staining showed that,compared with control group and shamed-kindled group, kindled female rats had enlarged ovaries with numerous growing follicles, espically many preantral follicles, and the total number of follicles and atretic follicles increased(P<0.05). The ovarian in study group exhibited polycystic changes. Ultrastructure of growing follicles of kindled rats exhibited increase of apoptotic granulose cells, theca cell hyperplasia, and more secretory granules can be found in theca cells, their ovarian exhibited polycystic changes. Conclusions Seizures initiated in amygdala result in polycystic ovary and loss of normal estrus cycle. Seizures initiated in amygdala result in polycystic ovary,growing follicles showed more apoptosis and increase of the secretion in the theca cell hyperplasia .更多还原