【作者】 刘义文；

【导师】 胡建国；

【作者基本信息】 宁夏医科大学 ， 内科学， 2011， 硕士

【摘要】 目的研究转化生长因子β1(Transforming Growth Factorβ1)在胃癌、胃不典型增生及正常胃组织中的表达及其与胃癌的发生、发展、侵袭及转移的关系。方法采用免疫组织化学(immunohistochemistry,IHC)S-P法检测56例胃癌组织、30例胃不典型增生(轻-中度)组织和20例正常胃组织中的TGF-β1的表达水平,分析在胃癌发生、发展不同阶段TGF-β1表达变化及TGF-β1与胃癌患者临床病理特征之间的关系。应用SPSS16.0统计软件包,根据资料类型分别采用χ~2检验、非参数秩和检验进行统计学分析处理,检验显著性水准取P=0.05,以P<0.05为差异有统计学意义。结果1 TGF-β1阳性表达呈棕黄色颗粒,主要定位于细胞质,细胞核中偶见散在的表达。正常胃组织、胃不典型增生(轻-中度)组织和胃癌组织中TGF-β1的总阳性表达率分别是10%(2/20)、43.3%(13/30)、83.9%(47/56),差异有显著性意义(H =45.46,P<0.01)。2 TGF-β1在胃癌分化程度较差组中的表达高于分化程度较好组中的表达,差异有统计学意义(u=-2.26,P=0.024),在浆膜浸润组中的表达高于无浆膜浸润组中的表达,差异有统计学意义(u=-3.47,P=0.001),在有淋巴结转移组中的表达明显高于无淋巴结转移组中的表达,差异有显著性意义(u=-3.87,P<0.001),在临床分期(TNM)为Ⅲ+Ⅳ期组中的表达高于Ⅰ+Ⅱ期组的表达,差异有统计学意义(u=-3.19,P=0.001);TGF-β1的表达与胃癌患者的性别、年龄及胃癌发生部位均无相关性(P>0.05)。结论1 TGF-β1可能在胃癌的发生和发展过程中起重要作用。2 TGF-β1有可能作为胃癌前病变发展为胃癌的预警因子。3 TGF-β1的表达与胃癌的恶性生物学行为有一定关系,TGF-β1可能成为评估胃癌预后的有效指标。更多还原

【Abstract】 Objective To investigate the expression of Transforming Growth Factorβ1 (TGF-β1) in the gastric carcinoma,atypical hyperplasia (low-moderate) of gastric mucosa and normal gastric mucosa tissue and the role of TGF-β1 in the gastric carcinoma development,invasion and metastasis.Methods The expression of TGF-β1 was measured by S-P method of immunohistochemical technique in 56 patients with gastric carcinoma,30 patients with atypical hyperplasia (low-moderate) of gastric mucosa and 20 normal gastric mucosa tissues.We investigated the changes of TGF-β1 expression in three groups and the ralationship between TGF-β1 and clinicopathological characteristics of gastric carcinoma;SPSS16.0 for windows software was used to analyse data.Difference of the data between groups was calculated byχ~2 test or nonparametric test. Significant level of test taking P=0.05,the significant difference was considered when P<0.05.Results1 The positive expression of TGF-β1 was brown granule, TGF-β1 was mainly expressed in cytoplasm,occasionally scattered expressed in nucleus.The positive rate of TGF-β1 expression increased significantly in gastric carcinoma tissues(83.9%) compared with that in atypical hyperplasia (low-moderate) of gastric mucosa tissues(43.3%) and that in normal gastric mucosa tissues,with a significant difference(H =45.46,P<0.01).2 The expression of TGF-β1 in patients with poorly differentiated cancers was significantly higher than those in well differentiated cancers(u=-2.26,P=0.024);The expression of TGF-β1 in serosa invasion group was significantly higher than those in no serosa invasion group(u=-3.47,P=0.001); The expression of TGF-β1 in lymph nodes metastatic group was significantly higher than those in no lymph nodes metastatic group (u=-3.87,P < 0.001); The expression of TGF-β1 in clinical stageⅢ+Ⅳgroup was significantly higher than those in clinical stageⅠ+Ⅱgroup (u=-3.19,P=0.001).The expression of TGF-β1 had no correlation with sex,age and different part of gastric carcinomas(P>0.05).Conclusions1 TGF-β1 probably play an important role in the origin and delepoment of gastric cancer.2 TGF-β1 as a possible precancerous lesion to gastric cancer warning factor.3 The expression of TGF-β1 is closely correlated with the malignant biologic behavior of gastric carcinoma, TGF-β1 may become an effective clinic prognostic index of gastric carcinoma.更多还原