【作者】 金银女；

【导师】 吴光； 崔松彪；

【作者基本信息】 延边大学 ， 内科学， 2011， 硕士

【摘要】 目的：本实验利用长期双侧颈总动脉结扎法制备血管性痴呆(Vascular dementi, VD)大鼠模型,并观察大鼠海马齿状回(Dentate gyrus, DG)区细胞外液中几种氨基酸含量的变化,探讨血管性痴呆对大鼠DG区氨基酸的影响。方法：本实验将成年Wistar大鼠(250-300g)随机分成对照组(n=6)、假手术组(n=6)和模型组(VD组)(n=6)。对照组不做手术,假手术组只剥离双侧颈总动脉但是不结扎,模型组剥离并永久性结扎双侧颈总动脉。术后30天开始,采用Morris水迷宫进行学习记忆的行为学观察；训练结束后,利用脑内微量透析法和高效液相色谱法测定海马DG区细胞外液中的7种氨基酸类物质(包括天门冬氨酸、谷氨酰胺、谷氨酸、甘氨酸、牛磺酸、丙氨酸和γ-氨基丁酸)的含量。结果：1.VD组与假手术组、对照组相比,水迷宫实验的逃避潜伏期明显延长,具有统计学意义(均为P<0.05),假手术组和对照组之间无明显差异(P>0.05)。2.VD组海马DG区细胞外液中的谷氨酰胺、牛磺酸、丙氨酸和γ-氨基丁酸的含量均明显高于对照组和假手术组,具有统计学意义(均为P<0.05)。其余三种氨基酸无明显变化(均为P>0.05)；假手术组的海马DG区七种氨基酸含量与对照组相比无明显差异(均为P>0.05)。结论：1.采用双侧颈总动脉结扎术能有效制备VD模型;2.VD可能与海马DG区谷氨酰胺、牛磺酸、丙氨酸和γ-氨基丁酸含量增加有关。更多还原

【Abstract】 Objective:To discussed the effect of vascular dementia on amino acids levels in hippocampal Dentate gyrus (DG) region, the vascular dementia (VD) rat model was prepared with permanent bilateral carotid artery ligation, and the extracellular concentrations of several amino acids in hippocampal DG region were observed in the VD rats.Methods:Adult Wistar rats (250～300g) were randomly divided into control group (n=6), sham operation group (n=6) and model group (VD group) (n=6). Control group without operation, sham operation group only bilateral carotid artery dissection but not ligation, model group stripping and permanent bilateral carotid artery ligation.30 days after the surgery, learning and memory behavior were observed using Morris water maze; after training, the extracellular concentrations of several amino acids(including aspartic acid, glutamine, glutamic acid, glycine, taurine, alanine and y-aminobutyric acid) in hippocampal DG region were determined by using brain microdialysis and high performance liquid chromatography techniques.Results:1. VD group compared with the sham group and the control group, water maze escape latency was prolonged, with statistical significance (P< 0.05, respectively), no significant difference between sham group and the control group (P > 0.05); 2. glutamine, taurine, alanine and y-aminobutyric acid levels were significantly higher than the control and the sham group, with statistical significance (P<0.05, respectively). No significant change in the other three amino acids (P> 0.05, respectively), and concentration of amino acids showed no significant difference between the control and the sham group (P> 0.05, respectively).Conclusion:1. Bilateral carotid artery ligation could effectively prepare VD model; 2. VD may be associated with enhancing of glutamine, taurine, alanine and y-aminobutyric acid in hippocampal DG region.更多还原