【作者】 王正华；

【导师】 郑军；

【作者基本信息】 三峡大学 ， 外科学， 2011， 硕士

【摘要】 目的:运用物元分析法,建立肝硬化疾病评分模型(SLCD),提出一种新的肝脏损害及肝功能储备评分公式,既SLCD评分公式。方法:采用复合因素法建立实验性大鼠肝硬化诱导模型,对肝硬化诱导过程中不同时相点大鼠肝组织损害程度和血清学肝功能储备变化进行动态观察。运用物元分析法获得实验性大鼠SLCD评分公式。结果:实验性大鼠肝硬化模型复制成功。选取年龄、血清总胆红素(TBil)、血清白蛋白(Alb)、血清前白蛋白(PA)、凝血酶原时间国际标准化比值(PT-INR)、血清肌酐(SCr)等6个检测指标,成功构建了实验性大鼠SLCD及SLCD评分公式。评估程度以数值(R值)表达,随着病变程度和纤维化程度加重,R值逐渐降低。R=1表示肝脏组织正常;0.702≤R<1表示肝脏炎症反应和局灶性变性、坏死;0.542≤R<0.702表示肝脏出现纤维组织增生现象;0.352≤R<0.542有假小叶形成,为肝硬化期;R﹤0.352表示肝硬化后期。结论:SLCD评分公式能较敏感而准确地反映实验性肝硬化大鼠肝脏损害及肝功能储备情况,若能在临床进一步证实其运用价值,则可为临床早期预防及治疗肝硬化疾病提供参考依据。更多还原

【Abstract】 Objective To construct the scoring model of liver cirrhosis disease(SLCD), to find a new score formula which was the SLCD score formula for the liver impairment and function reserve with the Matter-Element Analysis method.Methods To construct the rat liver cirrhosis model of experimental rat with composite factor method. In different phases of the rat liver cirrhosis model, to give a serological detection to the dynamic changes of liver impairment degree and liver function reserve, and a histological observation to the liver pathological alteration. To obtain the SLCD score formula by using the Matter-Element Analysis method.Results The experimental rat liver cirrhosis model was successfully copied. The experimental rat SLCD and SLCD score formula were successfully constructed with six detection indexes which were weeks, TBil, Alb, PA, PT-INR, SCr. The score was expressed by R, with the pathological changes and fibrosis were aggregated, R was gradually decreased. R=1, it meant the liver tissue was normal; 0.702≤R<1, it meant there were inflammatory reaction, focal degeneration and necrosis in liver; 0.542≤R<0.702, it meant there was fibroplasia in liver; 0.352≤R<0.542, pseudolobules were formed and it was in the liver cirrhosis stage. R﹤0.352, it meant later stage of liver cirrhosis.Conclusions The SLCD score formula was comparatively sensitive and exact in reflecting the liver impairment and liver function reserve of the experimental rat liver cirrhosis, if there would be clinical evidence on the application value, then it may be helpful for the early clinical prevention, diagnosis and treatment for the cirrhosis disease.更多还原