【作者】 丁晧；

【导师】 裘俊红；

【作者基本信息】 浙江工业大学 ， 化学工程， 2010， 硕士

【摘要】 藻蓝蛋白是螺旋藻的主要组成成分,广泛应用于食品、化妆品、医药等行业。藻蓝蛋白的生理活性如抗氧化、抗肿瘤、抗病毒等特性已被不断证实,使之更具广阔的应用前景。反胶束水合萃取技术是将反胶束萃取和水合物生成耦合在一起形成的一项新型、有发展潜力的分离技术,可应用于生物物质的活性控制及提取。反胶束水合萃取藻蓝蛋白在保证藻蓝蛋白活性的同时完成萃取和反萃取过程,操作较为简单。本文构建了CTAB/(正辛烷-正戊醇)反胶束体系,通过研究此体系中水合物生成对其内的藻蓝蛋白的萃取作用及纯化效果,获得反胶束水合萃取藻蓝蛋白的动力学规律以及温度、压力、CTAB浓度和初始含水量W0等对反胶束水合萃取藻蓝蛋白的影响规律,为进一步开展水合物法生物质活性控制及反胶束水合萃取技术的研究提供依据。纯水体系和反胶束体系中乙烯水合物对比实验表明:两种体系中水合物生成动力学特性差异较大,乙烯在反胶束体系和纯水体系中溶解度、溶解速率和体系中水含量的差别是造成反胶束体系和纯水体系中乙烯水合物生成动力学特性区别的本质所在。藻蓝蛋白的反胶束水合萃取实验表明:乙烯水合物的生成动力学曲线与不含藻蓝蛋白的反胶束中的生成动力学曲线基本相似。研究表明,在初始温度3.0℃,初始压力4.0 MPa,CTAB浓度0.05 mol/L,初始含水量40的条件下,水合萃取藻蓝蛋白的萃取率E可达81.3 %,表明该条件时的萃取效果比较好。由于水合萃取实验中气液比较大,所以在一定范围内初始温度和压力对萃取效果影响不大,属于非显著性因素,而较大的反胶束浓度和初始含水量有利于提高藻蓝蛋白萃取率。更多还原

【Abstract】 Phycocyanin, as one of the main components in Spirulina platensis, has been applied in food, cosmetic and medicine industries, etcSome important physiological activities such as antioxidant,antitumor and antiviral effect are generally identified,thus phycocyanin has wider application prospect.The new and innovative separation technology named as“reverse micellar hydrated extraction technology”, which integrated hydrate separation with reverse micellar extraction, is used for the control of biological activity and extraction of bio-product. The kinetic research was implemented on the extraction of phycocyanin from the reverse micellar system, which was formed by CTAB,n-octane and n-pentanol. Correspondingly,the extraction behavior of phycocyanin from the reverse micellar system as well the effect mechanism of temperature,pressure,CTAB concentration,and initial water content W0 on phycocyanin extraction were acquired. The results contributed new information for the further research on the control of biological activity and reverse micellar hydrated extraction of bio-product.Contrast experiments of ethylene hydrate formation in pure water system and reverse micellar system showed that the kinetics of hydrate formation had large difference between two systems,which was caused by the different solubility,dissolution rate of ethylene and the water content of two systems.The reverse micellar hydrated extraction experiments showed that, kinetic curves of ethylene hydrate formation in the reverse micellar system containing phycocyanin were similar to those of the reverse micellar system not contained phycocyanin. The maximum extraction yield of 81.3 % was obtained at initial temperature of 3.0℃, initial pressure of 4.0 MPa, CTAB concentration of 0.05 mol/L and initial water content of 40. Because of the large gas liquid ratio for the reverse micellar hydrated extraction experiments,the initial temperature and pressure were non-significant factor effecting extraction yield. And the increased CTAB concentration and initial water content favored the extraction yield of phycocyanin.更多还原