【作者】 张艳玉；

【导师】 高国兰；

【作者基本信息】 南昌大学 ， 妇产科学， 2011， 硕士

【摘要】 目的：利用人卵巢上皮性癌荷瘤裸鼠建立卵巢癌不良心理应激模型,探讨不良心理应激对人卵巢上皮性癌荷瘤裸鼠血清可溶性白介素2受体(sIL-2R)、血管内皮生长因子(VEGF)、CA-125影响。方法：将24只裸鼠随机分为4组：正常生长组(Ⅰ)、单纯应激组(Ⅱ)、单纯荷瘤组(Ⅲ)、荷瘤+应激组(Ⅳ),每组6只,建立相应的人卵巢上皮性癌荷裸鼠模型和不良心理应激模型。观察皮下瘤生长情况、裸鼠体重的变化,采用酶联免疫吸附法(ELISA)检测各组裸鼠血清中sIL-2R,CA-125,VEGF的含量。结果：Ⅳ裸鼠皮下瘤比Ⅲ生长较快,肿瘤增长率达66.33%。Ⅱ裸鼠血清中sIL-2R、VEGF水平与Ⅰ相比有显著性差异(P<0.05), sIL-2R、VEGF水平明显高于对照组(P<0.05)；Ⅱ裸鼠血清中CA-125水平与Ⅰ相比无显著性差异(P>0.05)。Ⅲ与Ⅰ,Ⅳ与Ⅰ相比sIL-2R、VEGF、CA-125水平均有显著性差异(P<0.05)。结论：1、成功建立了不良心理应激束缚模型。2、不良心理应激使人卵巢癌荷瘤裸鼠肿瘤的瘤体重量明显增加。不良心理应激可导致裸鼠血清中血清中的SIL-2R、VEGF、CA125明显升高,说明不良心理应激可抑制荷瘤裸鼠的免疫功能,可能促进肿瘤快速增长。更多还原

【Abstract】 Objective:To establish dys-psychological stress model using nude mice bearing human epithelium ovarian carcinoma.To investigate expression of Soluble Interleutin-2 Receptor(sIL-2R), vascular endothelial growth factor(VEGF) and CA-125 in human ovarian carcinoma of adverse psychological stress in nude mice.Methods:All nude mice were randomized into 4 groups(each n=6):normal control group(Ⅰ),simple stress group(Ⅱ),tumor group(Ⅲ),tumor plus stress group(Ⅳ).Adverse psychological stress model and human ovarian carcinoma xenografs model were established in correlated groups. The growth of tumors were observed in nude mice.The weight of subcutaneous tumor were measured,the concentrations of sIL-2R,VEGF and CA-125 in serum were detected by enzyme-linked immunoabsorbent assay (ELISA).Results:Compared with tumor group,the tumor grew faster in tumor plus stress group,the growth rate of which was 66.33%(P<0.05).The level of sIL-2R,VEGF in simple stress group was significantly higher than in normal control group(P<0.05). There was not significant difference in the expression of CA-125 level between simple stress group and normal control group (P>0.05).Tumor group and normal control group, tumor plus stress group and normal control group were significantly different when they were compared with correlated group(P<0.05),the concentrations of sIL-2R,VEGF and CA-125 in serum were significantly higher than correlated control group (P<0.05)Conclusions:1.Dys-psychological stress model with restraint was successfully established.2.The dys-psychological stress adds the tumor weight significantly.The level of sIL-2R,VEGF and CA125 in tumor plus stress groupwas significantly higher than in normal control group.Adverse psychological stress can suppress the immune function of tumor-bearing nude mice, resulting in rapid growth of tumors.更多还原