【作者】 赵俭；

【导师】 王世军；

【作者基本信息】 山东中医药大学 ， 中西医结合基础， 2011， 硕士

【摘要】 目的:探索寒性药物复制大鼠虚寒证模型的造模及模型评价方法。通过观察热性中药附子对虚寒证模型大鼠能量代谢及相关指标的影响,分析附子作用趋势、特点及规律,探寻反映实验动物寒热属性的客观指标,揭示其与寒热药物属性的内在联系,为更好的指导临床用药奠定基础。方法:用生石膏、龙胆草、黄柏和知母水煎液灌胃14天复制虚寒证大鼠模型,给予附子水煎液灌胃治疗7天,观察虚寒模型大鼠的一般状况、体温、寒热趋向等的变化;应用代谢笼收集大鼠24小时食量、饮水量、尿量和粪量;应用氧弹热量计检测大鼠摄入能、消化能、粪便能、可代谢能;采用酶联免疫吸附法检测大鼠血浆T3、T4、TSH、TRH含量;检测空腹血糖含量;检测肝组织糖原、乳酸含量、ATP酶活力、乳酸脱氢酶活力(LDH)、琥珀酸脱氢酶(SDH)活力;采用高效液相色谱法检测肝组织ATP含量及肝细胞能荷。结果:造模结束时模型组动物毛色污浊,腹泻明显,体重、自主活动及趾温明显降低,血浆丙酮酸、T3含量呈下降趋势。附子可以显著升高虚寒模型大鼠血浆T4含量及肝乳酸含量,明显提升肝乳酸脱氢酶和肝Na~+-K~+-ATP酶活力,显著升高肝细胞能荷;并且空腹血糖、体重、肛温、饮水量、尿量、在热箱的次数呈现一定的降低趋势;粪重、在寒箱的次数、单位体重摄入能、粪便能、单位体重粪便能,肝琥珀酸脱氢酶活力,ATP含量等指标呈现一定的升高趋势。结论:寒性药物生石膏、龙胆草、黄柏和知母配伍可成功复制大鼠虚寒证模型。热性中药附子能使虚寒模型大鼠能量代谢增强,改善大鼠虚寒状态,其药理作用的发挥同提高肝脏能量代谢和促进甲状腺激素的分泌关系密切。体重、体温、血浆甲状腺激素的含量、肝乳酸含量、ATP酶的活力、肝细胞能荷及空腹血糖含量等指标可以作为寒热证模型以及寒热性中药研究的重要评价指标。更多还原

【Abstract】 Objective: To explore methods of establishment and evaluation of deficiency-cold model in Rats, and to abserve the effect of monkshood, with heat properity acroding to the theory of TCM, on energy metabolism and on relevant indicators in deficiency-cold model in rats. To analyse the tendency and speciality of these effects, then find the signs of cold and heat, and reveal the inherent relationship to cold and heat properity of TCM. To provide a useful guidance for clinical medicine.Methods: In order to induce deficiency-cold model in rats, decoction of four Chinese medicinal herbs with cold property, gypsum, radix gentianae, golden cypress and rhizome anemarrhenae decoction were administered intragastrically to rats for 14 days. After the model was successfully produced, decoction of mankshood was given for 7 days for treatment. The effects on temperature and cold and heat preference in deficiency-cold model rats after drug administration were observed. Collecting the food, water, urine and dung in quantity of rats for 24 hours with metabolic cage .The EI, DE, FE and ME were detected by Oxygen Heat Calculation. T3, T4, TSH, TRH in blood serum were analyzed using Enzyme-linked immunosorbent assay. Fasting blood sugar, hepatic glycogen and LD levels were measured. The activities of hepatic ATPase, LDH and SDH in liver were analyzed using measuring kit respectively. The EC and the content of ATP in liver were dected using HPLC.Results: Rats in model group looked coloured and dirty, with obvious diarrhea. Body weight, autonomous activities and toe temperature reduced significantly. After the treatment with Mankshood, plasma pyruvate, T3 levels in blood serum decreased. T4 level in blood serum and hepatic LD level increased, and the activity of ATPase, LDH and EC in liver increased. The declining trend was shown in those indicators, such as fasting blood sugar, weight, water and urine in quantity. The rising trend was shown in those indicators, such as the activity of SDH, the content of ATP. Conclusions: Using gypsum, radix gentianae, golden cypress and rhizome anemarrhenae can successfully induce deficiency-cold model in rats. Mankshood can enhance energy metabolism in deficiency-cold model rats and improve the rat deficiency-cold state, the pharmacology mechanism may be related to increase the hepatic energy metabolism and thyroid hormone. Effective appraisal indicators of the cold and heat property of TCM include weight, temperature, cold and heat preference, thyroid hormone, EC, the activity of hepatic ATPase, LDH and SDH.更多还原