【作者】 陈慧慧；

【导师】 李贵海；

【作者基本信息】 山东中医药大学 ， 中药学， 2011， 硕士

【摘要】 目的:通过观察柴胡-黄芩不同比例配伍对解热抗炎作用的影响,阐明配伍对药对清热药效的影响;通过观察配伍对胆汁分泌的影响,找到最佳利胆作用的配伍比例;通过观察配伍对CCl₄所致肝损伤的保护作用,阐明配伍对药对疏肝解郁药效的影响。方法:按照柴胡和黄芩1:0,1:2,1:1,2:1,0:1的比例制备含生药1g/ml的受试药。采用2,4-二硝基苯酚和干酵母分别制备大鼠发热模型,混合致炎液制备小鼠耳肿胀模型,分别观察五种受试药液对两种大鼠发热模型和小鼠耳肿胀模型的影响;胆总管插管引流胆汁,测定给药后3h的胆汁分泌总量,计算胆汁的体重系数。测定胆汁中总胆红素,直接胆红素,胆固醇含量,并进行比较;采用CCl4制备小鼠肝损伤模型,分别观察五种受试药液对小鼠肝损伤模型肝指数、生化指标、病理组织学、免疫组织化学等主要指标的作用。结果:不同配伍比例受试药对2,4-二硝基苯酚致热大鼠模型的影响,与空白组比较,柴胡-黄芩1:2组和黄芩组解热作用显著,其它三组没有明显作用。对干酵母所致大鼠发热模型的影响与空白组比较,柴胡-黄芩1:1组、黄芩组、1:2组均具有明显的解热作用,2:1组和柴胡组无显著解热作用。不同配伍比例对小鼠耳肿胀度、耳肿胀率的影响:黄芩组和柴胡-黄芩1:2配伍组作用较为明显,其它三组作用与空白组比较无显著性差异;与空白组比较,黄芩组和配伍1:1组胆汁分泌量最多,其次为1:2组和2:1组,单独柴胡组无明显促进胆汁分泌的作用。与空白组比较各给药组的总胆红素水平、直接胆红素和胆固醇水平有不同程度的改变;不同配伍比例受试药对肝损伤各检测指标表现出不同的药效学作用,其中柴胡-黄芩配伍1:2组保肝作用相对更为明显,其次为黄芩组,柴胡组没有表现出明显的保护肝损伤的作用。结论:不同配伍比例对于解热抗炎、利胆和保肝作用表现出不同的作用强度。对不同致热源所致的大鼠发热模型,其解热作用最佳配伍比例表现出一定的差异,对2,4-二硝基苯酚致热大鼠模型以1:2配伍最强,而对干酵母发热模型则以1:1配伍组表现较好。抗炎作用则主要与配伍中所含黄芩的量有关;利胆作用最佳配伍比例在柴胡-黄芩1:2和1:1之间最优;对肝损伤的保护作用,以1:2组药效学作用最为明显,其次是单独黄芩组,表明黄芩起主要保肝作用,而配伍柴胡后保肝作用更明显。更多还原

【Abstract】 Objective:To review the anti-pyretic and anti-inflammatory effects of Radix Bupleuri and Radix Scutellare in different compatibilities,and elucidate the relationship of compatibility and pharmacodynamics;To observe the effect on bile secretion of rats administered by Radix Bupleuri and Radix Scutellare in compatibility,and look for the best proportion on Cholagogic Action;To Review the effect of Radix Bupleuri and Radix Scutellare in compatibility on hepatic injury by CCl4,and elucidate the relationship of compatibility and pharmacodynamics further.Methods:Testing medicine of 1g/ml in accordance with the ratio 1:0,1:2,1:1,2:1,0:1 of Radix Bupleuri and Radix Scutellariae were prepared.We built rat febrile models with 2,4-dinitrophenol and dried yeast respectively and models of swelling ear in mice with mixed liquid induced inflammation.Researchers observed effects of the five testing medicine on rat febrile models and models of swelling ear in mice;Researchers detected choleresis and calculated bile-body weight after administered and formed fistula in surgery three hours,detected and compared the amount of total bilirubin,direct bilirubin and cholesterol in bile;The effects of five testing medicine on main indicators,such as liver index,biochemical indicator,histopathology and immunohistochemistry,were detected in acute hepatic injuried mouse models by CCl4 .Results:Compared with the blank,the effect of the testing medicine in different compatibility ratio on rat febrile model by 2,4-dinitrophenol is that Radix Bupleuri:Radix Scutellariae 1:2 and 0:1 had statistical significance,and the other three groups have no obvious statistical significance.But the effect of the testing medicine in different compatibility ratio on rat febrile model by dry yeast is that Radix Bupleuri:Radix Scutellariae 1:1,0:1 and 1:2 had obvious statistical significance while the other two had no effect.Radix Bupleuri:Radix Scutellariae 0:1 and 1:2 groups had an obvious effect while the other three groups had no effect compared with the blank on tumfaction degree of mice’s ears and the swelling rate;Compared with the blank,Radix Bupleuri:Radix Scutellariae 0:1 and 1:1 group had the most choleresis,1:2 and 2:1 group followed,1:0 group had no significant difference.The levels of total bilirubin,direct bilirubin and cholesterol in each drug administration group were changed in different degree;The pharmacodynamic effects of the testing medicine in different compatibility ratio was different in all investigations of hepatic injury.Radix Bupleuri:Radix Scutellariae 1:2 had the strongest hepatoprotective effect,Radix Bupleuri:Radix Scutellariae 0:1 followed while the 1:0 group had no significant conservancy effect on hepatic injury.Conclusion:The testing medicine in different compatibility ratio has different effect size on the anti-pyretic and anti-inflammatory effects,cholagogic action and hepatoprotective effect.The best compatibility ratio is different in rat febrile models caused by two pyrogen.Radix Bupleuri:Radix Scutellariae 1:2 has the strongest effect on rat febrile model by 2,4-dinitrophenol while 1:1 on rat febrile model by dry yeast.The anti-inflammatory effects are mainly related to the amount of Radix Scutellariae in compatibility;The best compatibility ratio is between Radix Bupleuri:Radix Scutellare 1:2 and 1:1 on cholagogic action;The pharmacodynamic effects of the testing medicine in different compatibility ratio are different in all investigations of hepatic injury.Radix Bupleuri:Radix Scutellariae 1:2 has the strongest hepatoprotective effect,Radix Bupleuri:Radix Scutellariae 0:1 followed,while the 1:0 group had no significant conservancy effect on hepatic injury.That indicated Radix Scutellariae had the main hepatoprotective effect in compatibility,but the effect is stronger after compatibled with Radix Bupleuri.更多还原