【作者】 杨扬；

【导师】 洪亚辉；

【作者基本信息】 湖南农业大学 ， 细胞生物学， 2011， 硕士

【摘要】 小果油茶(Camellia meiocarpa Hu.)是一种重要的木本油料树种,在我国长江以南地区广泛分布。本研究对西南地区小果油茶群体资源从脂肪酸组成与分子标记方面进行了系统评价,其主要结果如下：(1)利用SRAP标记对6个小果油茶群体的180份材料进行遗传多样性研究。从87对SRAP引物中共筛选出9对多态性理想的引物组合,共扩增出184条带,其中多态性条带184条,多态性位点比率高达100%。平均有效等位基因数为1.7603,180份小果油茶总Nei基因多样性指数为0.4279,平均Shannon信息指数为0.6183,表明小果油茶群体具有较丰富的遗传多样性,遗传变异主要分布在群体内,群体间变异相对较小(2)从小果油茶茶油脂肪酸成分的多重比较得出,群体间油茶的含油率变异较大,脂肪酸成分变异不显著。且脂肪酸成分与地区的地理、环境因子相关性不显著,其遗传性状是主要的影响因子。(3)运用分子标记与脂肪酸组成变异分析,结果显示二种方法揭示的小果油茶群体聚类结果较一致,表明这二种方法在评价小果油茶遗传多样性中的耦合性较好。本研究还通过小果油茶遗传多样性和遗传结构的分析提出了对该物种遗传多样性的保护策略。更多还原

【Abstract】 Camellia meiocarpa Hu is a is one of the important oil tree species in south China. In this study, population resources of Camellia meiocarpa Hu were systematically evaluated at fatty acid compositions and molecular marker.The main results are as following:1. The genetic diversity of Camellia meiocarpa Hu was analyzed by SRAP markers.9 primers selected from 87 candidates were applied to the amplification which produced 184 bands. Of them 184 bands (100%) were polymorphic. The average effective number of alleles. Nei’s gene diversity and Shannon’s information index were 1.7603, the genetic diversity of 180 Camellia meiocarpa Hu materials was high with Nei’s gene diversity of 0.4279 and Shannon’s information index of 0.6183,the mainly genetic variation of Camellia meiocarpa Hu existed in the populations.2. By Multi-comparison to the composition of tea oil-Fatty acid in Camellia meiocarpa Hu, the result that the variation of value of oilness was greater among tea oil while the variation of Fatty acid was limited was brought. The composition of fatty acid was not remarkable relevance with the Geographic and environmental Factors. The main impact factor was its Genetic Character.3. The result showed that the Groups clustering results of Camellia meiocarpa Hu were Inclined by means of MMs and analyzing the variation of Fatty acid. It showed that the Coupling of this two kinds of methods were better in evaluating the Genetic Diversity of Camellia meiocarpa Hu.Based on the genetic information available for the native Camellia meiocarpa Hu, some conservation strategies were proposed.更多还原