化石利胆汤对胆石成石基因B-UGT及CYP7A1mRNA表达的影响

【作者】 盛天骄；

【导师】 林一帆；

【作者基本信息】 辽宁中医药大学 ， 中西医结合临床， 2011， 硕士

【摘要】 目的:研究化石利胆汤对胆总管结石患者胆石成石基因B-UGT及CYP7A1mRNA表达的影响。材料与方法:2010年1月至2010年10月,在沈阳军区总医院中医科与内镜中心就诊的经ERCP术最终确诊为胆总管结石的患者100例,随机分为两组(n=50例)。实验组,在西医常规治疗的同时,给予化石利胆汤100ml 3/日口服,对照组,术后仅给予西医常规治疗。采用RT-PCR方法,检测患者术后第1天、服药后第7天、及第30天的胆红素尿苷二磷酸葡萄糖醛酸基转移酶(Bilirubin UDP-Glucronosyl Transferase,B-UGT)及人胆固醇7α羟化酶(Human Cholesterol 7α-Hydroxylase,CYP7A1)mRNA的表达。结果:实验组治疗后第7天的血B-UGT的mRNA表达值为1.34±0.055,第30天为2.14±0.019,第7天的CYP7A1的mRNA表达为1.139±0.037,第30天为1.890±0.118,均较治疗前明显升高,亦高于同期的对照组,而对照组无变化。两组比较具有统计学意义(P<0.05)。结论:化石利胆汤可以提高胆总管结石患者血中B-UGT及CYP7A1的mRNA的含量,从而增加游离胆红素及内源性胆固醇的代谢,抑制成石性胆汁的形成,对其能否起到防治结石的作用仍需长期大量临床观察。更多还原

【Abstract】 Purpose:To explore the effects of the genes shows of cholelithiasis B-UGT and CYP7A1 after the treatment of ERCP and the Huashilidan decoction.Material and method:One hundred patients had been check-up by ultrasound of the abdomen、CT scan-abdominal、magnetic resonance cholangiopancre atography,had been certified by with cholelithiasis undergoing ERCP,and had been done with EST、mechanical lithotripsy、basket removal of stone. After the operation, they were randomly divided into two groups averagely. One was the experiment group given Huashilidan decoction after ERCP.The other was the control group only given the normal therapy. We scale the genes shows of B-UGT and CYP7A1,at the first day, the seventh day and the 30th day after the therapy.Results:After the treatment, the genes shows of B-UGT and CYP7A1 were greatly higher than before of the experiment group. But the control group were not. P<0.05.Conclusion:Huashilidan decoction can improve the genes shows of B-UGTand activity of monoconjugated bilirubin,then to reduce the rates of gall-stone by intervene the genes shows of B-UGT and CYP7A1.更多还原