丹参联合骨髓间充质干细胞移植对心肌梗死区血管新生的影响

【作者】 李珊；

【导师】 陈岩；

【作者基本信息】 辽宁中医药大学 ， 中西医结合临床， 2011， 硕士

【摘要】 目的:探讨骨髓间充质干细胞移植、丹参干预骨髓间充质干细胞零天、七天静脉注射对急性心肌梗死后梗死区血管新生的影响。材料与方法:将70只兔随机分为正常组10只、造模组60只(根据预实验的最低造模成功率70%计算)。造模组心肌梗死模型复制成功后随机分为模型组、零天注射干细胞组、零天静脉注射丹参+MSCs组和第七天静脉注射丹参+MSCs组。每组10只,各组间体重差异无显著性(P>0.05)。(1)正常组:不予干预。(2)模型对照组:单纯造模。(3)静脉零天注射干细胞组:造模后即刻予干细胞混悬液2×10~6细胞一次性注射。(4)零天静脉注射MSCs+丹参组:急性心肌梗死形成后,马上予干细胞混悬液2×10~6细胞一次性静脉注射,同时按15.4mg/kg予配制的丹参注射液,日一次静脉滴注,连续10天。(5)七天静脉注射MSCs+丹参组:造模后予15.4mg/kg配制的丹参注射液,日一次静脉滴注,连续10天,第七天静脉注射MSCs组2×10~6细胞一次性静脉注射。结果:免疫组化CD31积分光密度值分析显示:与正常组比较,其他各组均降低,除零天干细胞+丹参组,其余组差别均有统计学意义(P<0.05)。与模型组比较,干细胞组、零天干细胞+丹参组、七天干细胞+丹参组的免疫组化CD31均有升高(P<0.05),但干细胞组、零天干细胞+丹参组、七天干细胞+丹参组组间免疫组化CD31数值差别不大,无统计学差异(P>0.05)。血管内皮生长因子(bFGF)、碱性成纤维细胞生长因子(VEGF)各组间无统计学差异(P>0.05)。结论:1.骨髓间充质干细胞静脉注射可以迁移至心肌梗死区及周围区。2.骨髓间充质干细胞移植可促进心肌梗死区及梗死周围区血管新生。3.丹参可增强骨髓间充质干细胞移植的促进血管新生的作用,但统计学没有差异。4.在零天和七天两个时间点,联合丹参进行干细胞移植注射组间无差异。更多还原

【Abstract】 An experimental study of Danshen intervention to Bone marrow-derived mesenchymal stem cells transplantation in the treatment of acute miocardial infarction about Angiogenesis.Purpose:To evaluate the SM intervention mesenchymal stem cells in zero-day, seven days of intravenous injection on rabbits with acute myocardial infarction infarction angiogenesis.Material and method:70 randomly divided into normal group, 10 rats in model 60(according to pre-experimental modeling minimum 70% success rate). MI model successfully reproduced in the model were randomly divided into model, zero-day injection of stem cell group, zero-day intravenous Danshen injection(SM) + marrow MSCs group and the seventh day intravenous SM + marrow MSCs group. N = 10 in each group no significant difference in body weight(P> 0.05).(1)normal group: no intervention,(2)model group: simple modeling,(3)intravenous injection of zero-day stem cells Group: After the suspension immediately to the stem cells injected with 2×10~6 cells,(4)Zero-day intravenous SM + MSCs group: after the formation of acute myocardial infarction, immediately to the stem cell suspension 2×10~6 cells single intravenous injection, while I prepared by 15.4mg/kg Danshen injection, on an intravenous drip for 10 days,(5)seven days intravenous injection of MSCs + SM group: After the preparation of Salvia to 15.4mg/kg injection on an intravenous drip for 10 days, the seventh day of intravenous injection of Danshen and bone marrow MSCs groups: acute formed 1 week after myocardial infarction, to the stem cell suspension 2×10~6 cells single intravenous injection.Results:CD31 immunohistochemical analysis of integrated optical density values:Compared with normal group were lower in other groups, in addition to zero Heavenly Stems Cells + SM group the other group were statistically significant(P <0.05), compared with model group, stem cell group, zero Heavenly Stems cells + SM group, seven days a stem cell + SM group had higher(P <0.05), stem cell group, zero Heavenly Stems cells + SM group, seven days a stem cell + SM group was no significant difference between groups. bFGF, VEGF was no significant difference among the groups.Conclusion:1.Point transplantation of bone marrow mesenchymal stem cells, can be moved totheinfarctzone.2.Bone marrow mesenchymal stem cell transplantation for myocardial infarction and surrounding area infarction angiogenesis.3.Danshen can enhance bone marrow mesenchymal stem cells promote angiogenesis, but there was no difference.4.In the zero-day and seven days of two time points stem cell transplantation combined with Danshen injection were no different.更多还原