【作者】 赵晨晨；

【导师】 承伟；

【作者基本信息】 辽宁医学院 ， 生药学， 2011， 硕士

【摘要】 目的研究络石藤中总黄酮的提取及纯化工艺;观察在最佳提取纯化工艺下得到的络石藤总黄酮对小鼠的抗炎镇痛作用。方法采用微波与超声联用辅助提取络石藤总黄酮,依次进行单因素实验和正交实验,应用紫外分光光度法测定络石藤总黄酮含量,并以其提取率为考察指标,运用统计学方法分析实验结果确定最佳提取工艺;采用大孔吸附树脂纯化络石藤总黄酮,通过静态-动态吸附解吸实验及纯化工艺的考察确定最优纯化树脂和最佳纯化工艺;通过Xyl致小鼠耳肿胀和CAR致大鼠足趾肿胀观察纯化后络石藤总黄酮抗炎作用;通过小鼠热板法和小鼠HAc扭体法观察其镇痛作用。结果应用微波与超声联用辅助提取法,按照最优提取工艺得到的络石藤总黄酮提取率高于传统提取方法,并能显著缩短提取时间;采用大孔吸附树脂纯化的络石藤总黄酮,其纯度可达到86.43%;纯化后的络石藤总黄酮在抗炎实验中具有显著的抑制小鼠耳肿胀和大鼠足跖肿胀的作用,具有明显的抗炎作用;在镇痛实验中,总黄酮能显著减少小鼠扭体次数,提高小鼠痛阈值,具有一定的镇痛作用。结论微波与超声波联用辅助提取络石藤总黄酮的方法是可行的。粗提物可经大孔吸附树脂有效的纯化,纯化后的络石藤总黄酮具有一定的抗炎镇痛作用。更多还原

【Abstract】 ObjectiveTo study the extraction and purification process of total flavonoids from Chinese Starjasmine Stem and anti-inflammatory and analgesic effect of total flavonoids in the best condition of extraction and purification process.MethodsThe single factor test and the orthogonal test were taken to study the best extraction conditions of total flavonoids from Chinese Starjasmine Stem by microwave-assisted method and ultrasonic wave cooperated method through measuring the extraction rate of the total flavonoids by UV spectrophotometry and using statistical Methods for analysis of experimental results. Macroporous adsorptive resins was used in puriting total flavonoids from Chinese Starjasmine Stem for confirming the optimal purification resins and the best purification process through the static-dynamic adsorption and desorption experiments and purpification experiment. The anti-inflammatory effect of total flavonoids from Chinese Starjasmine Stem was researched using ear edema induced by xylene and paw edema caused by carrageen; and analgesic effects of Chinese Starjasmine Stem were observed in pain models of mice induced by acetic acid or hot plate. ResultsThe extraction rate of the total flavonoids from Chinese Starjasmine Stem using the best extraction conditions were higher than the rate of using traditional method by microwave-assisted method and ultrasonic wave cooperated method and could significantly shorten the extraction time. The purity of total flavonoids could reach 86.43% in the best condition of extraction and purification process. The total flavonoids has inhibitory effect on the swelling of mouse ear piece and rat paw edema. The total flavonoids from Chinese Starjasmine Stem have analgesic effects on the two pain models.ConclusionsThe method of microwave and ultrasonic extraction in the best condition was feasible. Absorbent resin could effictively increase the purity of total flavonoids. The puritied total flavonoids from Chinese Starjasmine Stem have anti-inflammatory and analgesic effects.更多还原