【作者】 王立建；

【导师】 东方阳； 李洪杰；

【作者基本信息】 河北科技师范学院 ， 作物遗传育种， 2011， 硕士

【摘要】 本研究利用小麦品种中国春(Chinese Spring, CS)与小麦D基因组供体粗山羊草(Aegilops tauschii)TA2424杂交,杂交后取其幼胚进行组织培养得到杂种F1代,F1再用中国春连续回交两代,回交后代自交四代得到2000多份BC2F4后代群体家系。通过检测杂交后代群体中粗山羊草的染色体片段导入情况,构建一套普通小麦中国春—粗山羊草染色体片段导入系(CSSLs)。选取小麦D基因组上的250对SSR标记,利用中国春和粗山羊草TA2424筛选出170对在两个亲本间具有多态性的SSR标记。用随机选取的238个杂交后代BC2F4的DNA作为模板进行PCR扩增,检测在杂种后代中两个亲本染色体之间的代换情况。在170对多态性引物中,有83%在杂交后代中检测到粗山羊草染色体导入片段,导入片段基本覆盖了D基因组,根据SSR检测的结果,绘制了一套覆盖率在95%以上的D基因组的染色体片段导入系图谱。在有些后代品系中,扩增产物带型和两个亲本的带型均不一致。在238个家系中片段代换变异率在90%以上的有2个标记位点:Xgpw322和Xgwm212;60%~90%的有4个标记位点:Xgwm106、Xcfd35、Xgwm114b和Xgwm624;0.42%~5.46%的有13标记个位点,分别分布在D基因组的不同染色体上。更多还原

【Abstract】 Over 2000 BC2F4 progeny lines were produced by crossing Chinese Spring wheat with Aegilops tauschii accession TA2424 via embryo rescue technique and backcrossing with the recurrent parent Chinese Spring. A set of chromosome segment substitution lines (CSSLs) was developed by detecting the chromosome segments of Ae. tauschii with the polymorphic SSR markers from D genome. Two hundred and fifty SSR markers were used for screening polymorphism between CS and TA2424. One hundred and seventy polymorphic SSR markers were selected by detecting the PCR amplification products on 8% non-denaturing polyacrylamide gel electrophoresis. Two hundred and thirty-eight BC2F4 randomly selected progeny lines were used for detecting chromosome segments of Ae. tauschii with the polymorphic SSR markers. Based on the results of SSR detection, a profile of CSSLs covering 95% of Ae. tauschii D genome was developed. The results of this study indicateed that the sizes of the amplified products were not comparable to either parent in some progeny lines. Among the 238 tested, two markers Xgpw322 and Xgwm 212 had over 90% of substitution frequency; Xgwm106, Xcfd35, Xgwm114b and Xgwm624, 60~90%; and 13 markers, 0.42~5.46%. These markers with variation were distributed on different chromosomes of D genome.更多还原