【作者】 帕丽旦·麦麦提；

【导师】 甫拉提·吐尔逊；

【作者基本信息】 新疆医科大学 ， 生理学， 2011， 硕士

【摘要】 目的：本研究在建立维医异常黑胆质性糖尿病病症大鼠模型基础上运用基于核磁共振的代谢组学技术,对异常黑胆质证、糖尿病、异常黑胆质性糖尿病病证大鼠及正常大鼠血清和尿液的代谢组分加以分析、判断及发现差异性生物标记物,探讨异常黑胆质性糖尿病病证的物质代谢网络,探索异常黑胆质在糖尿病发生发展过程中的病理生理基础及作用。方法：根据维吾尔医学理论建立维吾尔医异常黑胆质性糖尿病病证模型,对其血清和尿液进行核磁共振氢谱检测,对采集谱图采用OPLS-DA分析进行数据解读。通过皮尔森相关系数显著性差异检测及二维谱技术确定各组大鼠血清和尿液中的差异性代谢成分。结果：不同大鼠血清和尿液1HNMR代谢谱存在差异,血清中有VLDL、LDL、不饱和脂肪酸、丙酮、乙酰乙酸、丙酮酸、柠檬酸、甘氨酸、丙氨酸、酪氨酸、苯丙氨酸、亮氨酸、异亮氨酸、瓜氨酸、蛋氨酸、肌酸、肌醇、鲨肌醇、尿素等差异性代谢物出现(P<0.05),不同大鼠尿液差异性代谢物有柠檬酸、胍基乙酸、酪氨酸、蛋氨酸、赖氨酸、苯丙氨酸、色氨酸、异亮氨酸、缬氨酸、丙氨酸、天冬氨酸、肌酸酐等(P<0.05)。结论：异常黑胆质证组、糖尿病组、异常黑胆质性糖尿病病证组、正常对照组大鼠血清和尿液间存在差异代谢组分,异常黑胆质证的发生、发展过程中脂代谢紊乱,糖尿病大鼠存在糖代谢及能量代谢障碍。糖代谢紊乱、肌酸合成障碍等是糖尿病大鼠与异常黑胆质性糖尿病病症大鼠共同的病理生理过程,不同组大鼠间代谢组分差异在尿液中更明显。更多还原

【Abstract】 Objective: based on the establishment of Abnormal savda disease rat model with Diabetes Mellitus,appling the metabonomics technology through nuclear magnetic resonance (NMR),, analyse and discover the biological marker by Metabonomics study of Serum and urine of Abnormal savda model group,diabetes Mellitus model group, Abnormal savda disease carring Diabetes Mellitus model group and control group,investigate the substance metabolism network of Abnormal savda disease earring Diabetes Mellitus, explore the Pathophysiological background and function in the process of Abnormal savda disease carring Diabetes Mellitus.Methods: According to the theory of Uighur traditional medicine, Abnormal savda disease carring Diabetes Mellitus rat model was established, A 1H NMR based metabonomic analysis was performed on these serum and urine samples. A multi-steps date preprocessing procedure was completed and then Orthogonal Partial Least-Squares Discriminant Analysis(OPLS-DA) was fulfilled to distinguish the metabolic phenotypes, the influential biomarkers accounting for the metabolic signature perturbation were discovered by Pearson’s product moment correlation coefficient and 2D NMR.Results: It was found that between the H NMserum 5’-nucleotidase of four group exist signification difference, the variability of metabolin in the serum of four group were VLDL, LDL, unsaturated fatty acid, acetone, acetoacetic acid, pyruvic acid, citric acid, glycine, alanine, tyrosine, Phe acid, leucine, isoleucine, citrulline, methionine, creatine, inositol, shark inositol, urea,etc. (P<0.05), the variability of metabolin in the urine of four group were citric acid, guanidine acetic acid, tyrosine, methionine, lysine, phenylalanine, tryptophan, isoleucine, valine, alanine, aspartic acid, creatinine, etc. (P<0.05).Conclusion: the metablolite in the serum and urine of those Abnormal savda, diabetes, Abnormal savda disease carring Diabetes Mellitus and control group were significant differences.the development of abnormal savda can cause the metabolism disorganization of lipid.the glycolysis and energy synthesis metabolisim disorder in the Diabetes group.glycometabolic disturbance, creatine dyssynthesis are the congenerous Pathophysiological process of Abnormal savda and diabetes group.the sianificant difference of the variability of metabolin in the urine is more than the sianificant difference of the variability of metabolin in the serum.更多还原