【作者】 单莺；

【导师】 李新国；

【作者基本信息】 中南大学 ， 妇产科， 2010， 硕士

【摘要】 目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和层粘连蛋白受体(lmainin recepetor,LN-R)在卵巢癌发生和发展中的作用和临床意义。方法用免疫组织化学SP法检测19例正常卵巢组织、23例良性卵巢肿瘤及42例卵巢癌组织中VEGF和LN-R的表达,用卡方检验和Sperman相关分析统计结果,计算出P值。分析其表达与肿瘤病理类型、组织学分级、FIGO临床分期、有无转移的关系。结果1.VEGF在正常卵巢组织、良性卵巢肿瘤及卵巢癌中的阳性表达分别为10.5%、43.4%及73.8%。良性卵巢肿瘤与正常卵巢组织中VEGF的阳性表达率有显著差异(P<0.05),卵巢癌与良性卵巢肿瘤中VEGF的阳性表达率有显著差异(P<0.05)。2.VEGF在卵巢浆液性囊腺癌中的阳性表达率为76%,在卵巢非浆液性囊腺癌中阳性表达率为70.6%。两组VEGF阳性表达率无显著性差异(χ2=0.153,P>0.05)；VEGF在低分化组中的阳性表达率为90.9%,明显高于高中分化组的阳性表达率55%(χ2=6.98,P<0.05)；VEGF在Ⅲ期-Ⅳ期中的阳性表达率为86.9%,显著高于Ⅰ期-Ⅱ期VEGF阳性表达率57.8%(χ2=4.54,P<0.05)；VEGF在伴有淋巴结或大网膜转移的卵巢癌组织中阳性表达率86.4%,明显高于不伴有淋巴结或大网膜转移的卵巢癌组织VEGF的阳性表达率55%(χ2=5.05,P<0.05)。3.LN-R在正常卵巢组织中不表达,在良性卵巢肿瘤中阳性表达率为4.3%,在卵巢癌中的阳性表达率为76.2%。LN-R在卵巢癌与良性卵巢肿瘤中的阳性表达率有显著差异(P<0.05)。4.LN-R在卵巢浆液性囊腺癌中的阳性表达率为76%,在卵巢非浆液性囊腺癌中阳性表达率为76.5%。两组LN-R的阳性表达率无显著性差异(x2=0.001,P>0.05)；LN-R在低分化组阳性表达率为90.9%,明显高于高中分化组的阳性表达率60%(x2=5.51,P<0.05)；LN-R在Ⅲ期-Ⅳ期中阳性表达率为91.3%,显著高于Ⅰ期-Ⅱ期中的阳性表达率57.8%(x2=6.40,P，0.05)；LN-R在伴有淋巴结或大网膜转移的卵巢癌组织中阳性表达率为81.8%,明显高于不伴有淋巴结或大网膜转移的卵巢癌组织中的阳性表达率50%(x2=4.77,P<0.05)。5.卵巢癌中VEGF的表达与LN-R的表达有相关性(P<0.05)。结论1.VEGF和LN-R在卵巢肿瘤的发生、发展过程中起着一定的作用。2.两指标与卵巢癌的临床期别、癌细胞分化程度以及有无大网膜、淋巴结转移有关,提示VEGF和LN-R可作为临床上预测卵巢癌预后的指标。3.在卵巢癌中VEGF和LN-R表现出一定的相关性,在卵巢癌发生和发展中呈协同作用。提示两者在卵巢肿瘤的发生发展中可能有着较为密切的联系。更多还原

【Abstract】 Objective:To explore the role and clinical significance of vascular endothelial growth factor(VEGF)) and lmainin recepetor (LN-R) in ovarian cancer.Methods:Using method of Immunohistochemical SP, the expression of VEGF and LN-R in 19 cases of normal ovarian tissues,23 cases of benign ovarian tumors and 42 cases of ovarian cancer were detected. Statistical analysis was performed with chi-square test and Sperman correlation.The relationship of their expression and pathological type, histological grade, FIGO stage, whether having metastasis were analysised.Results:1. The positive expression of VEGF in normal ovarian tissues and benign ovarian tumors and ovarian cance were 10.5%,43.4% and 73.8%,respectively.The positive expression rate of VEGF was significantly different in normal ovarian tissue and benign ovarian tumor tissue (P<0.05) and also was significantly different in benign ovarian tumor and ovarian cancer.(P<0.05)2. The positive expression rate of VEGF in ovarian serous adenocarcinoma was 76%,that in other ovarian cancers was 70.6%. The difference of VEGF positive expression rate of the two groups was unsignificant (χ2=0.153, P>0.05); The positive expression rate of VEGF in the poorly differentiated group (90.9%) was significantly higher than that in the high differentiated group (55%) (χ2=6.98, P<0.05);The rate of positive expression of VEGF in StageⅢ-Ⅳ(86.9%) was significantly higher than that in the stageⅠ-Ⅱ(57.8%) (χ2=4.54, P<0.05); In the ovarian cancer tissues with lymph nodes or omentum metastasis,the VEGF positive expression rate was 86.4%, significantly higher than that without metastasis (55%)(χ=5.05, P<0.05)3. LN-R did not express in normal ovarian tissue,LN-R expressed in benign ovarian tumors with low level (4.3%),with high levels of expression in ovarian cancer (76.2%). The positive expression rate of LN-R was significantly different between benign ovarian tumor and ovarian cancer.(P<0.05)4. The positive expression rate of LN-R in ovarian serous adenocarcinoma was 76%, that in other ovarican cancer was 76.5%. LN-R positive expression rate of the two groups had no significant difference (χ2=0.001, P>0.05); The positive expression rate of LN-R in the poorly differentiated group (90.9%)was significantly higher than that in the high differentiated group(60%) (χ2=5.51, P<0.05); The positive expression of LN-R in stageⅢ-Ⅳ(91.3%) was significantly higher than that in the stageⅠ-Ⅱ(57.8%) (χ2 =6.40, P<0.05); The positive expression rate of LN-R in ovarian cancer with lymph nodes or omentum metastasis(81.8%) was significantly higher than that without lymph nodes or omentum metastasis (50%)(χ2=4.77, P<0.05) 5.VEGF and LN-R show some positive correlation in the occurrence and development of ovarian cancer. (P<0.05)Conclusions:1. VEGF and LN-R play a role in the occurrence and development of ovanan cancer.2. The positive expression of VEGF and LN-R has relationship with clinical stage and histological grading, lymph nodes or omentum metastasis, and has no relationship with pathological type; which indicated that VEGF and LN-R could predict prognostics of ovarian cancer.3. VEGF and LN-R show some correlation in the occurrence and development of ovarian cancer, which indicated that they cooperated in the occurrence and development of ovarian cancer.更多还原