【作者】 陈碧仪；

【导师】 杨作成；

【作者基本信息】 中南大学 ， 儿科学， 2011， 硕士

【摘要】 目的探讨纤维蛋白原β链(Fgp)-148C/T及-455G/A基因多态性与川崎病(KD)发病及并发冠状动脉损伤(CAL)之间的关系。方法：应用聚合酶链反应-限制性内切酶片断长度多态性分析(PCR-RFLP)方法结合琼脂糖凝胶电泳技术,对47例川崎病患儿和60例正常对照组儿童进行纤维蛋白原β链-148C/T及-455G/A基因多态性分析。结果(1)KD组Fgp-148C/T基因多态性的CC、CT、TT基因型分布频率和C、T等位基因频率与正常对照组比较差异无显著性(χ2=4.194、2.64,P均>0.05)。合并CAL组CT基因型明显高于无CAL组,差异有显著性(χ2=7.009,P<0.05)。合并CAL组和无CAL组等位基因频率比较差异无显著性(χ2=0.862,P>0.05)；(2)KD组Fgp-455G/A基因多态性的GG、GA、AA基因型分布频率和G、A等位基因频率与正常对照组比较差异无显著性(χ2=4.194、2.64,P均>0.05)。合并CAL组GA基因型明显高于无CAL组,差异有显著性(χ2=7.009,P<0.05)。合并CAL组和无CAL组等位基因频率比较差异无显著性(χ2=0.862,P>0.05)结论(1)Fgp-148C/T(?)和-455G/A基因型和等位基因频率与KD的发生均无明显关联性。(2)Fgp-148C/T基因的CT基因型与KD合并CAL的发生存在关联。(3)Fgp-455G/A基因的GA基因型与KD合并CAL的发生存在关联。更多还原

【Abstract】 Objective To investigate the genetic polymorphisms of children with Kawasaki Disease (KD) and that complicated with coronary artery lesion (CAL) in theβ-Fibrinogen gene-148C/T (Fgβ-148C/T) and theβ-Fibrinogen gene-455G/A(Fgβ-455G/A).Methods 47 cases of patients with KD and 60 healthy children as controls were studied in this research. Polymorphism analysis of these genes (Fgβ-148C/T and Fgβ-455G/A respectively) were detected with polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP).Results (1) For theβ-Fibrinogen gene-148C/T, there were no significant differences between genotype frequency of CC, CT and TT types in KD and healthy controls (χ2=4.194, P>0.05). There was no significant difference between allele frequency of C and T types in KD and controls (χ2=2.64, P>0.05). The CT genotype in KD patients with CAL is significantly higher than that without CAL (χ2=7.009, P<0.05). There were no significant difference between allele frequency of C and T types in KD complicated with CAL and without CAL (χ2=0.862, P> 0.05). (2) For theβ-Fibrinogen gene-455G/A, there were no significant differences between genotype frequency of GG, GA and AA types in KD and healthy controls (χ2=4.194, P>0.05). There was no significant difference between allele frequency of G and A types in KD and controls (χ2=2.64, P>0.05). The GA type in KD patients with CAL is significantly higher than that without CAL (χ2=7.009, P<0.05). There were no significant difference between allele frequency of G and A types in KD complicated with CAL and without CAL (χ2=0.862, P>0.05).Conclusions (1) The polymorphisms of the (3-Fibrinogen gene-148C/T and theβ-Fibrinogen gene-455G/A had not been found association with the pathogenesis of KD. (2) The polymorphisms of CT type of theβ-Fibrinogen gene-148C/T might be related with KD complicated with CAL. (3) The polymorphisms of GA type of theβ-Fibrinogen gene-455G/A might be related with KD complicated with CAL更多还原