【作者】 周嫔婷；

【导师】 肖波；

【作者基本信息】 中南大学 ， 神经病学， 2011， 硕士

【摘要】 目的研究TRPC3 (transient receptor potential canonical 3)、TRPC6 (transient receptor potential canonical 6)在难治性颞叶癫痫患者颞叶皮层中的表达,探讨其在难治性颞叶癫痫发病中的作用。方法收集20例难治性颞叶癫痫患者作为实验组,6例急性颞叶脑外伤患者作为对照组。取手术切除颞叶皮层组织为研究对象。采用免疫组化及Western blot方法定性、定量检测TRPC3、6在各组颞叶皮层组织中的表达。结果免疫组化染色显示TRPC3、6主要位于神经元胞膜与胞浆内；与对照组相比,实验组TRPC3、6阳性细胞数显著增多,差异具有统计学意义(P<0.05)。Western blot检测显示TRPC3、6分别在相应的分子量97KDa及106KDa处出现蛋白条带；与对照组相比,实验组TRPC3、6与β-actin的灰度比值显著增加,差异具有统计学意义(P<0.05),这与免疫组化的结果一致。结论TRPC3、6在难治性颞叶癫痫患者颞叶皮层组织中表达上调,提示TRPC3、6可能参与了难治性颞叶癫痫的发病。更多还原

【Abstract】 Object To investigate the expression of transient receptor potential canonical 3(TRPC3) and transient receptor potential canonical 6(TRPC6) in the temporal cortex from patients with intractable temporal lobe epilepsy(TLE) and explore their potential roles in epileptogenesis.Methods This study was based on 20 patients with intractable temporal lobe epilepsy and 6 controls with acute head trauma.Temporal cortex tissue was obtained from each subject. Immunohistochemistry and Western blot were performed to examine the expression of TRPC3 and TRPC6 in each group.Results The immunohistochemisty study showed that TRPC3 and TRPC6 were mainly located in the cytomembrane and cytoplasm of neurons. Immunohistochemistry and Western blot analyses showed increased expression of TRPC3 and TRPC6 in patients with intractable TLE compared with controls.Conclusions The expression of TRPC3 and TRPC6 was upregulated in the temporal cortex tissue of patients with intractable TLE, which suggests their potential roles in epileptogenesis.更多还原