【作者】 欧圣；

【导师】 黄小红；

【作者基本信息】 福建农林大学 ， 基础兽医学， 2011， 硕士

【摘要】 以“芪苓1号”、“芪苓2号”和“抗球虫中药”作为添加剂,研究其对艾维茵肉鸡生产性能、抗氧化能力、免疫功能、血液生化指标、消化酶的影响。将1日龄艾维茵肉鸡2000只随机分为阴性对照组、阳性对照组、中药1、2、3组,每组4个重复,每个重复100只。阴性对照组饲喂基础日粮,阳性对照组在基础日粮中添加杆菌肽锌30ppm和硫酸抗敌素6ppm,中药1、2、3组除饲喂基础日粮外,第1~3周分别通过饮水添加1.4g/L、2.8g/L、2.8g/L芪苓1号,第4周至出栏添加0.8g/L、1.5g/L、1.5g/L芪苓2号,中药3组全程添加抗球虫中药。结果表明:“芪苓1号”、“芪苓2号”可以降低试验肉鸡料重比,中药2、3组试验肉鸡的屠宰率、胸肌率和腿肌率显著高于阴性对照组(P<0.05)。中药1、2、3组的试验鸡血清中SOD的活性极显著地高于阴性对照组(P<0.01);中药2、3组的试验鸡血清中总抗氧化能力极显著高于阴性对照组和阳性对照组(P<0.01);中药3组试验鸡血清中丙二醛的含量极显著低于阴性对照组和阳性对照组(P<0.01)。中药2、3组的IgA、IgG、C3、ALT、白蛋白、总蛋白含量均高于阴性对照组(P<0.05)。中药1、2、3组、阳性对照组血清溶菌酶活性极显著高于阴性对照组(P<0.01)。中药1组的试验鸡小肠淀粉酶的活性极显著高于阴性对照组(P<0.01)。中药3组试验鸡肠道内容物淀粉酶的活性极显著高于阴性对照组和阳性对照组(P<0.01)。中药1、2、3组和阳性对照组的试验鸡小肠中脂肪酶的活性极显著高于阴性对照组(P<0.01)。中药1、3组试验鸡小肠内容物中蛋白酶的活性极显著高于阳性对照组(P<0.01)。高剂量的“芪苓1号”、“芪苓2号”可以提高肉鸡的生产性能和屠宰性能,降低生产成本,提高肉鸡的免疫能力。将1日龄艾维茵肉鸡216只随机分为空白对照组、感染不给药组、磺胺氯丙嗪钠组、复方中药低、中、高剂量组,每组3个重复,每个重复12只,除空白对照组外,其它各组于14日龄经口感染8X10~4球虫卵囊,第15日龄时通过饮水饲喂抗球虫药。空白对照组与感染不给药组只饲喂基础日粮,磺胺氯丙嗪钠组饮水添加1.0 g/L磺胺氯丙嗪钠可溶性粉,复方中药低、中、高剂量组分别饮水添加0.8g/L、1.2g/L、1.6g/L抗球虫中药。研究抗球虫中药对试验肉鸡的成活率、增重、病变值、卵囊值、抗球虫指数及盲肠的病变情况的影响。结果表明,在四个给药组中,高剂量组的抗球虫指数为180.13,磺胺氯丙嗪钠组的抗球虫指数为181.06,表明高剂量抗球虫中药与磺胺氯丙嗪钠均对球虫敏感;其中高剂量中药组的试验肉鸡存活率达到97.22%,相对增重率达到90.41%,表明高剂量的抗球虫中药具有较强的抗球虫效果;中药低剂量组、中剂量组的ACI值分别为170.67、166.11,它们均达到了对球虫敏感的水平,也具有中等抗球虫水平。以常山、柴胡、白头翁、钩藤、仙鹤草、地榆组成的抗球虫中药具有抗球虫效果,其中高剂量抗球虫中药与磺胺氯丙嗪钠一样,具有明显的抗球虫效果。更多还原

【Abstract】 As a feed additive for broiler trial,the "Qi Ling 1 ", "Qi Ling 2 " and "anticoccidial Chinese medicine" were used to study the influence for broiler production,antioxidant activity,immune function,blood biochemical indexes,digestive enzymes activity. Two thousands 1-day broilers were randomly divided into the negative control group, the positive control group, Chinese medicine1,2,3 groups, with 4 replications per group and 100 broilers per replication. Negative control group was fed the basal diet,the positive control group was fed the basal diet with 30ppm zinc bacitracin and 6ppm Colistin Sulphate,Chinese medicine1,2,3groups were fed the basal diet with“Qi Ling 1”from first week to third week(Doses were 1.4g/L,2.8g/L,2.8g/L,by drinking water),and with“Qi Ling 2”from forth week to slaughter(Doses were 0.8g/L,1.5g/L,1.5g/L,by drinking water),Chinese medicine 3 group was fed by anticoccidial Chinese medicine in the whole process. The results showed that: The "Qi Ling 1 ", "Qi Ling 2 "could reduce feed conversion ratio of broilers,the dressing percentage eviscerated rate,breast muscle rate and leg muscle rate of the Chinese medicine group 2 and group 3 were higher than the negative control group,the difference were significant (P<0.05). SOD activity of Chinese medicine group 1,2,3 were significantly higher than the negative control group (P<0.01);the total antioxidant capacity of Chinese medicine group 2 and group 3 were significantly higher than the negative group and positive control group (P<0.01);the MDA of Chinese medicine group 3 was significantly lower than the negative control group and the positive control group(P<0.01). The IgA,IgG,C3,ALT,albumin and total protein levels of Chinese medicine group 2 and group 3 were higher than the negative control group (P<0.05). Serum lysozyme activity of Chinese medicine group 1,2,3 and the positive control group were significantly higher than the negative control group (P<0.01). The amylase activity of Chinese medicine group 1 was significantly higher than the negative control group (P<0.01), the intestinal contents amylase activity of Chinese medicine group 3 was significantly higher than the negative control group and positive control group (P<0.01). The small intestine lipase activity of Chinese medicine group 1,2,3 and positive control group were significantly higher than the negative control group (P<0.01). The protease activity from intestinal contents of Chinese medicine group 1 and group 3 were significantly higher than the positive control group (P<0.01).The high dose "Qi Ling 1 ", "Qi Ling 2 " could increase the production of broiler performance and slaughter performance, reduce production costs and improve immunity of broilers.Two hundred and sixteen 1-day broilers were randomly divided into blank group, infection without drug group, sodium sulfa chlorpromazine group, low, medium and high dose Chinese medicine group, with 3 replications per group and 12 broilers per replication. Except the blank group, the other groups were infected 8×10~4 coccidia oocysts by mouth, and were fed anticoccidial drugs by drinking water on the fifteenth day. Blank group and infection without drug group were fed basal diet, sodium sulfa chlorpromazine group was fed sulfonamide sodium soluble powder chlorpromazine 1.0 g/L by drinking water, low, medium and high dose Chinese medicine group were fed anticoccidial Chinese medicine 0.8g/L,1.2g/L,1.6g/L. The "Anticoccidial Chinese medicine" was used to study the influence of survival rate, relative growth rate, disease value, oocysts values, anti-coccidiosis cecal lesion score and the pathology of the cecum. The results showed: in the four dose groups, the anticoccidial index of high dose group was 180.13, the anticoccidial index of sulfa chlorpromazine group was 181.06, this showed that high dose Chinese medicine and the sulfa chlorpromazine were sensitive to coccidiosis; the survival rate of Chinese medicine high-dose group reached to 97.22%, and its relative growth rate was 90.41%, this showed that the Chinese medicine had a strong effect against coccidiosis; the ACI values of low dose groupand middle dose group were 170.67and 166.11, both of them had reached to the level of coccidia-sensitive,and also had moderate level of resistance to coccidiosis. Anticoccidial Chinese medicine composited with Radix Dichroae, RootofChneseThorowax, Anemone chinensis,Uncaria macrophylla Wall, Agrimonia eupatoria, Radix Sanguisorbae, had the effect for anticoccidial. The high doses anticoccidial Chinese medicine had the same effect for anticoccidial as the sodium sulfa chlorpromaze.更多还原