【作者】 郑春生；

【导师】 陈炯；

【作者基本信息】 安徽医科大学 ， 外科学， 2011， 硕士

【摘要】 目的:探讨C3(补体3)、C4B1(补体4B1)、ApoE(载脂蛋白E)在胰腺癌中的表达及其与TNM分期和胰腺癌淋巴转移的相关性。方法:将标本分为胰腺癌组和正常胰腺组,依据淋巴结转移情况将胰腺癌组分为有淋巴结转移组和无淋巴结转移组,依据TNM分期将胰腺癌组分为Ⅰ期组和Ⅱ-Ⅳ期组。分别应用免疫组织化学(二步法)、逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction,RT-PCR)和蛋白质印记(Western Blot)技术检测各组标本C3、C4B1、ApoE表达的差异性。结果:C3、C4B1、ApoE在胰腺癌中均呈高表达;C3与肿瘤的TNM分期和有无淋巴结转移无相关性;C4B1、ApoE与肿瘤的TNM分期和有无淋巴结转移密切相关:Ⅱ-Ⅳ期胰腺癌和有淋巴转移的胰腺癌中的表达明显高于Ⅰ期胰腺癌和无淋巴转移的胰腺癌。结论:C3、C4B1、ApoE在胰腺癌中均呈高表达;C3与肿瘤有无淋巴转移及TNM分期无关,说明C3只参与胰腺癌的早期事件,可能成为胰腺癌的早期诊断标记物;C4B1、ApoE与肿瘤发展密切相关,反映胰腺癌的生物学行为,可作为胰腺癌进展期预测指标。更多还原

【Abstract】 Objectiv: To investigate the association of C3 , C4B1 and ApoE expressions with TNM stage and lymph node metastasis of human pancreatic cancer.Methods:The specimens were divided into groups of pancreatic cancer group and normal pancreas, pancreatic cancer according to lymph node metastasis group was divided into lymph node metastasis and without lymph node metastasis, according to the TNM stage of pancreatic cancer group was divided into groupsⅠandⅡ-Ⅳof Group. Immunohistochemistry (two-step), Reverse Transcriptase Polymerase Chain Reaction,(RT-PCR) and Western Blot were detected by imprint technique C3, C4B1, ApoE expression differences.Results: C3, C4B1, ApoE overexpressed in pancreatic cancer, C3 was not related with tumor’s lymph node metastasis and TNM stage, C4B1 and ApoE wer closely related with tumor’s lymph node metastasis and TNM stage: expression of C4B1 and ApoE in pancreatic cancer those with lymph node metastasis and stageⅡ-Ⅳwere significantly higher than those without lymph node metastasis and stageⅠ.Conclusion: C3, C4B1, ApoE overexpressed in pancreatic cancer, C3 is not related with tumor’s lymph node metastasis and TNM stage, indicating that C3 is only an early event in pancreatic cancer, may be markers for early diagnosis of pancreatic cancer, C4B1, ApoE is closely related to tumor development, reflecting the biological behavior of pancreatic cancer and can be used as predictors of advanced pancreatic cancer.更多还原