【作者】 朱伟政；

【导师】 叶茂昌； 王来平； 李容新；

【作者基本信息】 安徽医科大学 ， 口腔临床医学， 2011， 硕士

【摘要】 研究目的近年来在骨组织工程学领域出现了很多种新的组织工程骨支架材料修复骨缺损的研究方法,如组织工程支架材料复合生长因子法及组织工程支架材料复合带蒂预购血管肌瓣法等等。本研究采用骨组织工程支架材料联合血管内皮细胞应用于骨缺损修复,观察探讨组织工程骨细胞材料复合体应用于骨缺损修复时细胞生长分化成骨及骨血管再生的状况。意图通过实验研究的方法来更好地解释组织工程骨促血管再生和促成骨的机制。研究方法应用酶消化法,抽取兔自身长骨骨髓,体外分离、培养扩增骨髓基质干细胞至第三代,加入条件培养基后诱导分化为血管内皮细胞,培养传代后,接种于无机活性元素支架材料上,构成组织工程骨细胞材料复合体,移植到兔股骨的人工缺损上,组织工程骨细胞材料复合体移植为实验组,单纯无机活性元素支架材料移植为对照组。通过采用免疫组织化学染色,术后X线定量检测等方法追踪在组织工程骨模型中,骨缺损局部骨量及血管内皮生长因子(VEGF)表达量的变化,观察术后兔股骨的修复成骨及血管再生情况,并将结果进行对比研究。结果双侧骨缺损术后定期观察均可见成骨细胞密集,骨组织修复,血管再生重建;免疫组化结果显示实验组早期成骨速度及血管新生速度方面优于对照组,X线检测亦显示实验组缺损区术后同期骨量及外在骨痂X线阻射程度高于对照组。表明实验组在成骨方面及血管再生方面相对于对照组均具有一定优势。结论无机活性诱导元素骨支架材料在用于修复骨缺损时能有效促进成骨细胞生长、分化,提高其成骨特性,而细胞复合组织工程骨修复支架材料在骨缺损修复中的应用,提高了无机活性诱导元素骨支架材料在分化成骨及血管再生方面的优势,使骨缺损的修复及重建效率得到有效提升。更多还原

【Abstract】 PurposeBone tissue engineering in recent years there are many kinds of new bone tissue engineering scaffold material of bone defect repair methods, such as tissue engineering scaffold material of Growth Factor and tissue engineering scaffold materials futures vascular pedicle muscle flaps, etc. . In this study, the joint bone tissue engineering scaffold material used in vascular endothelial cells of bone defects, tissue engineering of bone cells observed composite materials used in bone repair cells to differentiate into bone and bone status of angiogenesis. Experimental research methods intended to better explain the proliferation of vascular tissue engineering of bone regeneration and promote the mechanism of bone.MethodsThe enzyme digestion, extraction of rabbit’s own long bone marrow, isolate, culture expansion of bone marrow stromal cells to the third generation, joined the conditioned medium after the induction of differentiation into vascular endothelial cells, culture and passage were inoculated in inorganic active elements of the scaffolds to form Tissue engineering of bone cell materials composite, ported to the artificial femur defects on composite tissue engineering of bone cells transplanted as the experimental group, the simple transfer of inorganic materials as active elements stent control group. By using immunohistochemical staining, after X-quantitative detection method for tracking the tissue engineering of bone model, the bone defect the local expression of bone mass and changes in VEGF was observed after the repair of femur bone and vascular regeneration, and the results were compared.ResultsRegularly observed after bilateral bone osteoblasts were seen dense bone tissue repair, angiogenesis reconstruction; Immunohistochemistry showed that the experimental group of early osteogenesis and angiogenesis speed speed than the control group, X ray detection experiment also shows group of bone defects after year and the external callus higher level of X radiopacity. Show that the terms of the experimental group and the blood vessels in the bone regeneration compared with the control group had certain advantages.ConclusionsActivity induced by inorganic elements of bone scaffolds in repairing bone defects when used effectively promote bone cell growth, differentiation and enhance its osteogenic characteristics, and cells combined with tissue engineering scaffold material for bone repair in the repair of bone defects and improve the inorganic activity induced bone scaffold elements to differentiate into bone and blood vessels in the regeneration of the advantages, so that the repair and reconstruction of bone defects effectively improve efficiency.更多还原