【作者】 任艳兵；

【导师】 熊奇如；

【作者基本信息】 安徽医科大学 ， 外科学， 2011， 硕士

【摘要】 目的检测5-LOX和survivin在胆囊癌中表达情况;探讨5-LOX和survivin两种蛋白表达是否具有相关性;结合临床资料,了解5-LOX和survivin与临床资料的关系,为胆囊癌的早期诊断和治疗提供理论依据。方法标本来自我院肝胆外科自2000年6月至2009年6月行手术切除治疗且病理诊断明确的32例胆囊癌患者的组织标本,详细记录患者的临床资料。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-peroxidase,S-P)法检测32例胆囊癌及相应的癌旁组织和20例正常对照慢性胆囊炎组织中5-LOX和survivin蛋白的表达状况,结合临床资料进行统计学分析。结果癌组织中5-LOX和survivin高表达均明显高于相应的癌旁组(P <0.05;P <0.05)和正常对照组,癌旁组与正常对照组相比,差异也具有显著性(P =0.001;P =0.001)。癌组织和癌旁组织中5-LOX和survivin的表达均存在正相关(P <0.05;P <0.05)。结论5-LOX和survivin与胆囊癌的形成关系密切。5-LOX和survivin与胆囊癌形成可能存在协同作用。更多还原

【Abstract】 Objective Detemining the expression of 5-LOX and survivin. Analyze the expression of 5-LOX and survivin in gallbladder carcinoma and their relationship with clinical date. To elucidate the mechanisms for early diagnosis and clinical application.Methods The specimens were taken from32 patients with gallbladder carcinoma in An Hui Medical Afflicated Hospital from june 2006 to june 2009 Record the clicical date in detail. The expression of 5-LOX and survivin was assayed by Streptavidin-peroxidase immunohistochemistry in 32 gallbladder carcinomas and adjacent non-cancerous tissues,20 normal gallbladder tissue as control. Experimental date were analyzed together with the clinical pathological datum.Results The expression of 5-LOX and survivin in gallbladder carcinomas was significantly than adjacent non-cancerous tissues(P <0.05;P <0.05) and nomal control tissues,and there was significant difference between adjacent non-cancerous tissues and nomal control tissues(P =0.001;P =0.001).positive correlation were found between the expression of 5-LOX and survivin in gallbladder carcinomas and adjacent non-cancerous tissues(P <0.05;P <0.05).Conclusion 5-LOX and survivin play a important role in the formation of gallbladder carcinoma. There may be some extend coordinated function in the formation of gallbladder carcinoma.更多还原