【作者】 蔡成福；

【导师】 赵德安；

【作者基本信息】 福建医科大学 ， 耳鼻咽喉科， 2011， 硕士

【摘要】 背景与目的:鼻咽癌发病率居头颈部恶性肿瘤之首。近年来,大量研究表明,Wnt/β-catenin信号通路与鼻咽癌的发生、发展存在密切的关系。Wnt/β-catenin信号通路关键因子β-catenin与核转录因子TCF/LEF结合启动靶基因表达是该信号通路活化的关键步骤,而chibby是β-catenin抑制因子,通过对β-catenin的抑制阻止靶基因的表达,可能是一种潜在的肿瘤抑制因子。因此,本研究运用Real time PCR及免疫组织化学的方法检测鼻咽癌组织中chibby mRNA及蛋白的表达,探讨鼻咽癌组织中chibby mRNA及蛋白的表达水平及临床病理学意义。材料与方法:(1)2010.4-2011.01在我院门诊初诊确诊的符合入组条件的鼻咽鳞癌患者45例,取其病变部位活检标本及对侧正常鼻咽粘膜筛检标本共90份。(2)提取总RNA,反转录为cDNA,采用Real time PCR检测所有标本chibby mRNA的表达水平。(3)采用免疫组织化学方法检测所有标本chibby蛋白的表达水平。(4)测量鼻咽癌组织chibby mRNA及蛋白降低的发生率,及其与各临床特征的相关性。结果:(1)鼻咽癌组织chibby mRNA表达降低率为68.9%(31/45,95% CI 53%-82%)。Chibby mRNA在鼻咽癌组织中相对表达量低于正常鼻咽粘膜(0.0776±0.0059,0.0835±0.0056,P<0.05)。(2)鼻咽癌组织chibby蛋白表达降低率为73.3%(33/45,95% CI 58%-85%)。chibby蛋白在鼻咽癌组织中的阳性率低于正常鼻咽粘膜(42.2% 19/45,91.1% 41/45,P<0.05)。(3)chibby mRNA及蛋白表达水平与T分期及临床分期有相关(P<0.05),与性别、年龄、颈淋巴结转移无明显相关性(P>0.05)。结论:(1)chibby mRNA及蛋白在鼻咽癌组织中表达均降低;(2)chibby的表达水平与肿瘤T分期及临床分期相关,T分期及临床分期越高,chibby表达越低;同时未能发现chibby的表达与性别、年龄、颈淋巴结转移及WHO病理分型相关。更多还原

【Abstract】 Background and Objective:Nasopharyngeal carcinoma (NPC) is one of the common head and neck cancers, which incidence rate is in the first head and neck cancer. In recent years, a large number of studies have shown that there is a close relationship between wnt/β-catenin signaling pathway and the development of NPC.β-catenin ,a critical factor of Wnt/β-catenin signaling pathway ,combined with nuclear transcription factor TCF / LEF , starts with the expression of target gene. This is the critical step of the activation of signaling pathway. chibby ,aβ-catenin inhibitor, prevent expression of target gene through inhibition ofβ-catenin .It may be a potential tumor suppressor. We analyse the Chibby expression and its function in NPC.Material and Methods:Forty-five cases of NPC were included in this study. Tumour and matched contralateral mucosa were sampled. Chibby expression levels were investigated by quantitative RT-PCR, and immunohistochemical methods revealed correct expression patterns of Chibby protein. The chibby expression levels and expression patterns of Chibby protein in different groups of different characters were analysed.Result:The rate of low expressions of Chibby mRNA is in tumour 68.9% (31/45 95% CI 53%-82%), the expressions in the tumour and matched contralateral mucosa were 0.0776±0.0059 and 0.0835±0.0056(P<0.05). The rate of low expressions of Chibby protein in tumour is 73.3% (33/45, 95% CI 58%-85%), the positive rate in the tumour and matched contralateral mucosa were 42.2%(19/45) and 91.1%(41/45), P<0.05. The early tumour staging and the clinical staging had the low chibby expression levels and expression patterns of Chibby protein.Conclusion:There is low expression of Chibby mRNA and protein in NPC, The chibby expression levels and expression patterns of Chibby protein associate with tumour staging and clinical staging.更多还原