【作者】 吴秋婉；

【导师】 张志明；

【作者基本信息】 福建医科大学 ， 肿瘤学， 2011， 硕士

【摘要】 【目的】研究人乳腺癌中细胞外基质蛋白-1(ECM1)蛋白与mRNA的表达,以及ECM1蛋白的表达与微淋巴管密度(LMVD)的关系,揭示ECM1在人乳腺癌淋巴浸润和转移中的作用。【方法】1.免疫组织化学技术半定量检测41例患者乳腺癌组织及其癌旁正常乳腺组织、腋窝淋巴结ECM1蛋白表达情况,以D2-40标记淋巴管,计数LMVD,分析ECM1蛋白的表达与LMVD的关系。2.实时荧光定量聚合酶链反应检测以上患者相应组织ECM1 mRNA的相对表达量。3.结合临床病理资料,探讨ECM1与人乳腺癌淋巴管生成的关系及其在乳腺癌淋巴浸润和转移中的作用。【结果】1.乳腺癌组织LMVD(12.95±11.10个/400倍视野)高于正常乳腺组织(2.24±1.14个/400倍视野)及腋窝淋巴结(5.49±3.36个/400倍视野)(P<0.01);淋巴结转移阳性组的腋窝淋巴结LMVD(9.32±9.03个/400倍视野)高于阴性组腋窝淋巴结(2.18±1.50个/400倍视野)(P<0.01)。2.乳腺癌组织ECM1蛋白表达阳性率为75.6%(31/41),正常乳腺组织为9.8%(4/41),腋窝淋巴结为29.3%(12/41);乳腺癌组织ECM1蛋白表达阳性率高于相应的正常乳腺组织及腋窝淋巴结(χ2=39.91, P<0.01)。阳性组淋巴结ECM1蛋白表达阳性率63.2%(12/19)高于阴性组淋巴结(P<0.01)。乳腺癌组织ECM1 mRNA的相对表达量高于正常乳腺组织及腋窝淋巴结(P<0.01)。3. ECM1蛋白的表达与乳腺癌组织LMVD呈正相关(r=0.347, P<0.05)。4. ECM1蛋白的表达与患者绝经前后、病理类型、组织学分级、原发肿瘤大小及淋巴结转移情况无关(P>0.05)。【结论】1.乳腺癌组织中ECM1蛋白及mRNA的表达高于相应的正常乳腺上皮组织和腋窝淋巴结。2. ECM1蛋白的表达与乳腺癌组织LMVD呈正相关,可能通过参与淋巴管生成,促进人乳腺癌淋巴浸润和转移。3.淋巴管生成在乳腺癌细胞淋巴管播散中发挥重要作用,新生淋巴管是乳腺癌淋巴转移的主要通路。更多还原

【Abstract】 [Objective] To investigate the protein and mRNA expression of extracellular matrix protein-1 (ECM1) in human breast carcinoma and the correlation between ECM1 protein and lymphatic microvessel density (LMVD), and to reveal the roles of ECM1 in the lymphatic infiltration and metastasis of human breast carcinoma.[Methods] 1. The protein expression of ECM1 was examined in the cancerous tissues, their normal counterparts and axillary lymph nodes in 41 cases of breast carcinoma by immunohistochemistry. Lymph vessels were labeled by using D2-40 and LMVD was calculated. The correlation between the expression of ECM1 protein and LMVD was analyzed.2. The relative expression level of ECM1 mRNA was detected in the correspongding tissues of the above subjects by real-time fluorescent quantitative polymerase chain reaction.3. The clinicopathological factors were taken into account to analyze the correlation between expression of ECM1 and lymphangiogenesis in breast carcinoma, and the roles of ECM1 in the lymphatic infiltration and metastasis of human breast carcinoma.[Results] 1. LMVD in the carcinoma tissues (12.95±11.10 per 400x field of vision) was higher than that in the corresponding normal breast epithelial tissues (2.24±1.14 per 400x field of vision) and that in the axillary lymph nodes (5.49±3.36 per 400x field of vision) (P<0.01). LMVD in the axillary lymph nodes with lymph node metastases (9.32±9.03 per 400x field of vision) was higher than that without metastases (2.18±1.50 per 400x field of vision) (P<0.01).2. The positive rate of ECM1 protein expression in the breast carcinoma tissues, their normal counterparts and the axillary lymph nodes were 75.6%(31/41), 9.8%(4/41), 29.3%(12/41), respectively. The positive rate of ECM1 expression in the breast carcinoma tissues was higher than that in the corresponding epithelial tissues and axillary lymph nodes (χ2=39.91, P<0.01). The positive rate of ECM1 in positive lymph nodes (12/19, 63.2%) was higher than that in negative lymph nodes (P<0.01).The relative expression of ECM1 mRNA in the breast carcinoma tissues was higher than that in the corresponding epithelial tissues and axillary lymph nodes (P<0.01).3. The protein expression of ECM1 was positively correlated with LMVD in the breast carcinoma tissues (r=0.347, P<0.05).4. The protein expression of ECM1 in the breast carcinoma tissues didn’t correlate with premenopause or postmenopause, pathological features, histological classfication, size of primary tumor or status of lymph node metastasis (P>0.05, respectively).[Conclutions] 1. The expression of ECM1 protein and mRNA in the breast carcinoma tissues were both higher than those in the corresponding breast epithelial tissues and axillary lymph nodes.2. The protein expression of ECM1 was positively correlated with LMVD in the breast carcinoma tissues. ECM1 might faciliate lymphangiogenesis, to promote lymphatic infiltration and metastasis of human breast carcinoma.3. Lymphangiogenesis played a key role in the process of breast carcinoma cells disseminating to lymph vessels. The new-born lymph vessels were the main access for breast carcinoma cells to lymphatic metastasis.更多还原