【作者】 黄志云；

【导师】 姚新生；

【作者基本信息】 暨南大学 ， 药物化学， 2011， 硕士

【摘要】 目的对海洋放线菌Micromonospora sp. (No. M2DG17)以及Streptomyces sp. (No.0616121)中的细胞毒活性成分进行研究。方法采用活性追踪分离的方法,综合利用硅胶开放柱色谱、ODS中低压柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱等分离手段进行化合物的分离、纯化；利用理化性质和波谱学分析等方法对单体化合物进行结构鉴定,并对其进行活性评价。结果从海洋放线菌Micromonospora sp. (No. M2DG17)发酵物中分离得到了12个化合物,其结构分别为3-羟甲基-β-卡巴林(化合物1)、3-甲基-β-卡巴林(化合物2)、β-卡巴林(化合物3)、麦芽酚(化合物4)、环(L-脯-L-苯丙)二肽(化合物5)、环(L-脯-L-缬)二肽(化合物6)、环(L-脯-L-亮)二肽(化合物7)、环(L-脯-L-异亮)二肽(化合物8)、邻苯二甲酸二(2-乙基-己基)酯(化合物9)、邻苯二甲酸异丁酯(化合物10)、苯乙酸(化合物11)、大豆素(化合物12)。其中化合物1-5、7-10,为首次从该属放线菌中分离得到,化合物2显示出弱的HCT 116细胞生长抑制活性(IC50为65.0μM)；从菌株Streptomyces sp. (No.0616121)发酵物中分离得到9个化合物,其中4个化合物与化合物5-8结构相同,另外5个化合物的结构分别为邻苯二甲酸正丁酯(化合物13)、环(D-脯-L-缬)二肽(化合物14)、环(L-脯-L-甲硫)二肽(化合物15)、环(D-脯-L-异亮)二肽(化合物16)、环(丙-缬)二肽(化合物17)。其中化合物15为首次从该属放线菌中分离得到。更多还原

【Abstract】 Objective To study the bioactive secondary metabolites from two strains of marine-derived Actinomycetes Micromonospora sp. (M2DG17) and Streptomyces sp. (0616121). Methods By bioassay-guided fractionation, various chromatographic and spectroscopic methods were applied for the isolation and structural identification of pure compounds from the broth of Micromonospora sp. (M2DG17) and Streptomyces sp. (0616121). Results Twelve compounds were obtained from the broth of Micromonospora sp. (M2DG17) and their structures were identified as 3-hydroxymethyl-β-carboline (compound 1),3-methyl-β-carboline (compound 2), P-carboline (compound 3),3-Hydroxy-2-methyl-4-pyrone (compound 4), Cyclo-(L-Pro-L-Phe) (compound 5), Cyclo-(L-Pro-L-Leu) (compound 6), Cyclo-(L-Pro-L-Val) (compound 7), and Cyclo-(L-Pro-L-Ile) (compound 8), Phthalate(2-ethyl)hexyl (compound 9), Isobutyl phthalate (compound 10), Phenylacetic Acid (compound 11), and Daidzein (compound 12), respectively. Compound 1-5,7-10 were isolated from the genus of Micromonospora sp. (M2DG17) for the first time, and compound 2 showed weak inhibitory effect on HCT 116 cell lines with IC50 values of 65.0μM. Nine compounds were obtained from the broth of Streptomyces sp. (0616121) respectively., and four compounds were identical with compounds 5-10. The other five ones were identified as Dibutyi phthalate(compound 13), Cyclo-(D-Pro-L-Val) (compound 14), Cyclo- (L-Pro-L-Met) (compound 15), Cyclo- (D-Pro-L-Ile) (compound 16), Cyclo (Ala-Leu) (compound 17). Compound 15 were isolated from the genus of Streptomyces sp. (0616121) for the first time.更多还原