【作者】 池翠云；

【导师】 岑颖洲；

【作者基本信息】 暨南大学 ， 有机化学， 2011， 硕士

【摘要】 对瑶族植物药四方藤(Cissus Pteroclada Hayata)化学成分进行了较为系统的研究,从中分离和鉴定了9个单体化合物和1个混合物,分别是β-谷甾醇、豆甾醇和菜油甾醇的混合物(1),β-香树脂醇(2),白桦脂酸(3),β-谷甾醇(4),木栓酮(5),11-O-没食子酰岩白菜素(6),11-O-(4’-hydroxy benzoyl) bergenin(7),胡萝卜苷(8),没食子酸(9),岩白菜素(10)。除化合物10外,其余均为首次从该种植物中分离得到,化合物6、7为首次从该属植物中分离得到。采用水蒸气蒸馏(SD)法、超声萃取水蒸气蒸馏(UESD)法分别提取瑶药四方藤的挥发油,并应用GC-MS对所提取的挥发油进行分析比较。实验结果表明,水蒸气蒸馏法提取的挥发油鉴定了22种挥发性成分,鉴定的成分占挥发油总量的83.41%；超声萃取辅助水蒸气蒸馏法提取的挥发油鉴定出28种挥发性成分,鉴定的成分占挥发油总量的77.73%。两种方法相比,UESD法提取挥发油的得率约为SD法提取挥发油提取得率的2倍。从千斤拔(Flemingia philippinensis Merr.et Rolfe.)中分离并鉴定了5个单体化合物和1个黄酮混合物,分别是5-羟基-8-γ,γ-二甲代烯丙基-3’,3’-二甲基-吡喃并[6,7]色酮(Eriosematin,11)、羽扇豆醇(12)、染料木黄酮(13)、赝靛黄素和刺芒柄花素的混合物(14),3’-甲氧基香豌豆苷元(15)、5,7,3’,4’,5’-五甲氧基黄酮(16)。其中化合物15、16及混合物14均为首次从该植物中分离得到。采用MTT法研究了四方藤醇提物各萃取部位及部分化合物的抗肿瘤活性。实验结果表明,四方藤乙酸乙酯萃取部位、正丁醇萃取部位、6、7、11、13、15以及混合物14具有一定的抗肿瘤活性,其他组分及化合物不具有抗肿瘤活性。四方藤乙酸乙酯萃取物对鼻咽癌细胞CNE和宫颈癌细胞Hela具有较好的活性,其IC50分别为35.55μg/mL和39.74μg/mL。11-O-(4’-hydroxy benzoyl) bergenin及11-O-没食子酰岩白菜素对结肠癌细胞HT-29抑制作用较强,IC50分别是12.36μg/mL和16.33μg/mL。Eriosematin对宫颈癌细胞Hela的生长具有较强的抑制作用,其IC50为12.22μg/mL,并且随着化合物浓度的增加,抑制肿瘤细胞的增殖作用逐渐增强。更多还原

【Abstract】 The chemical constituents of Yao medicine Cissus Pteroclada Hayata were studied more systematically in this paper. Nine compounds and one mixture were isolated and identified to be the mixture ofβ-sitosterol, stigmasterol and campesterol(1), beta-amyrin(2), betulinic acid(3),β-sitosterol(4) friedelin(5),11-O-loylbergenin(6),11-O-(4’-hydroxy benzoyl) bergenin(7), daucosterol(8), gallic acid(9), bergenin(10). All the compounds had never been separated from this plant before except the compound 10. Compounds 6 and 7 were obtained from the plants of Cissus for the first time.Volatile oils were exacted by steam distillation(SD) method and ultrasound-assist-ted steam distillation(UESD) method from Yao medicine Cissus Pteroclada Hayata. The results showed that:22 volatile components were identified with the mothod of SD, which accounted for 83.41% of total essential oil.28 volatile components were identified with the mothod of UESD, which accounted for 77.73% of total essential oil. Compared two methods, the yield of volatile oil extracted by UESD was 2 times with its by SD.Five compounds and one mixture were isolated from Flemingia philippinensis Merr.et Rolfe and they were identified to be eriosematin(11), lupeol(12), genistein(13), the mixture of psedo-baptigenin and formononetin(14),3’-O-Methylorobol(15), 5,7,3’,4’,5’-pentamethoxyflavone(16). The compounds of 15,16 and mixture 14 were isolated from this plant for the first time.The anti-tumor activity of extract in Cissus Pteroclada Hayata and some compounds were studied by MTT method. The results showed that:ethyl acetate extract and butanol extract of Cissus Pteroclada Hayata together with compounds of 6, 7,11,13,15 and mixture 14 had some anti-tumor activity. But other compounds did not have anti-tumor activity. Ethyl acetate extract of Cissus Pteroclada Hayata had better activity in the inhibition of CNE and Hela cells. The IC50 values reached to 35.55μg/mL and 39.74μg/mL.11-O-(4’-hydroxy benzoyl) bergenin and 11-O-galloyl-bergenin showed apparent effect on the growth of cancer cells HT-29, its IC50 values reached to 12.36μg/mL and 16.33μg/mL. Eriosematin inhibitting the growth of cervical cancer Hela was outstanding, its IC50 value reached to 12.22μg/mL. And with increasing concentrations of Eriosematin, inhibition of tumor cells gradually increased.更多还原