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NF-кB在大鼠重症急性胰腺炎肾损伤发病机制中作用的实验研究
The Study of the Role of NF-кB in the Pathogenesis of Severe Acute Pancreatitis-associated Renal Injury in Rats
【作者】 张帮柱；
【导师】 莫小华；
【作者基本信息】 昆明医学院 ， 外科学， 2011， 硕士
【摘要】 【目的】探讨核因子-κB(nuclear factor-kappa B,NF-κB)在重症急性胰腺炎(SAP)肾损伤发病机制中的作用,探讨N-乙酰半胱氨酸(N-acety-L-cysteine,NAC)在SAP肾损伤中的治疗作用及其机制。【方法】54只SD大鼠随机分为假手术组(SO组),重症急性胰腺炎组(SAP组)及NAC治疗组(NAC组)各组18只。以5%牛磺胆酸钠溶液逆行注入胰胆管制作SAP模型。NAC组于造模后5min经阴茎背静脉注入NAC(200mg/kg)。分别于6h、12h、24h时间点随机处死大鼠。采用免疫组化法检测肾脏组织NF-κB和肿瘤坏死因子(TNF-a)活性,胰腺和肾脏组织做病理切片观察病理变化,同时行血清淀粉酶(AMY)、尿素氮(BUN)、肌酐(Cr)检查。【结果】SAP组造模后肾脏组织NF-κB和TNF-α显著升高,各时间点SAP组比SO组明显升高(P<0.05),NAC组比SAP组明显降低(P<0.05);NAC组AMY、BUN、Cr含量与SAP组相比明显降低(P<0.05)。【结论】肾脏组织NF-κB活化及TNF-α过度表达可能是SAP并发肾损伤的机制之一,NAC可能抑制肾脏NF-κB活性进而抑制TNF-α的表达,对SAP肾损伤具有一定的保护作用。
【Abstract】 [Objective]To investigate the effect of nuclear factor-kappa B (NF-κB) in the pathogenesis of pancreatitis-associated renal injury in severe acute pancreatitis and the therapeutical effect and mechanism of N-acety-L-cysteine (NAC) in pancreatitis-associated renal injury in severe acute pancreatitis.[Methods]Probabilistic Divide 54 SD rats into sham operated group, severe acute panreatitis group and NAC treated group. Injecting 5% sodium taurocholate into pancreatic duct to make SAP model. NAC treated group is to inject NAC (200mg/kg) into vena dorsalis penis 5min after the making of SAP model. Kill the rats at 6h、12h、24h respectively. Detect the activity of NF-κB and TNF-a in kidney by immunohistochemisty. Observe pancreas and kidney by pathological section. Detect scrum amylase. creatinine and urea nitrogen.[Result]The level of NF-κB and TNF-a in kidney in SAP group is significant high and the level of SAP group is higher than SO group, the level of NAC group is significant lower than SAP group. the level of scrum amylase. creatinine and urea nitrogen in NAC group is significant lower than SAP group (P<0.05) at each time point.[Conclusions]The activation of NF-κB and the over expression of TNF-αin kidney maybe one of the mechanism of pancreatitis-associated renal injury in severe acute pancreatitis. NAC may be able to inhibit the activation of NF-κB and then to inhibit the over expression of TNF-a in kidney. So NAC has some protective effect.