大鼠面神经损伤后面神经核团神经元凋亡的磁共振波谱成像研究

【作者】 张振光；

【导师】 田伟；

【作者基本信息】 昆明医学院 ， 医学影像学与核医学， 2011， 硕士

【摘要】 目的建立SD大鼠双侧面神经高位损伤的模型。通过高位切断SD大鼠双侧面神经主干,诱发双侧面神经核团的神经元凋亡,为磁共振1H-MRS活体检测脑内面神经元的凋亡提供实验基础。方法清洁级雄性SD大鼠20只,将其双侧面神经主干在茎乳孔处牵拉出约3mm后切断,并对大鼠术后进行面神经麻痹的评价。结果术后1-3周SD大鼠呈现出双侧面神经完全性麻痹的表现。结论SD大鼠双侧面神经高位损伤模型可导致双侧面神经完全性麻痹,且重复性好,可为磁共振1H-MRS活体检测脑内面神经元的凋亡提供实验基础。目的探讨利用磁共振氢质子波谱(1H-MRS)技术对大鼠双侧面神经高位损伤后的面神经核团内神经元凋亡进行活体观察分析的可行性。方法选SD大鼠24只,并将其分为正常对照组与模型组进行1H-MRS扫描。完成扫描后用GE 3.0T MR仪影像工作站Functool 4.5.5软件进行分析,获得N-乙酰天门冬氨酸(NAA)、肌酸(Cr)、胆碱(Cho)浓度进行测量。比较正常对照组与模型组的NAA、Cho、NAA/Cr、Cho/Cr、NAA/Cho差异有无统计学意义。结果模型组的NAA、NAA/Cr、NAA/Cho均低于正常对照组,差异有统计学意义,P值均小于0.05(模型组和正常对照组的NAA分别为7840.0±2671.46、10173.18±1500.33,t=3.159,P=0.003；模型组和正常对照组的NAA/Cr分别为0.94±0.33、1.27±0.14,t=3.782,P=0.001；模型组和正常对照组的NAA/Cho分别为1.46±0.39、1.76±0.45,t=2.076,P=0.046)。模型组的Cho、Cho/Cr均与正常对照组差异无统计学意义,P值均大于0.05(模型组和正常对照组的Cho分别为5514.50±1801.51、6035.76±1357.42,t=0.962,P=0.343；模型组和正常对照组的Cho/Cr分别为1.46±0.39、1.76±0.45,t=1.418,P=0.165)。结论大鼠双侧面神经高位切断后可导致面神经核团神经元的凋亡,利用1H-MRS技术可在活体上检测面神经核团内面神经元的凋亡情况。更多还原

【Abstract】 Objective To establish SD rats’ two sides of facial nerve high level injured model,we cut the SD rats’ two sides of facial nerve trunk on the high level, which Induced neurons apoptosis of two sides of facial nerve nuclear regiment, and provided experimental basis for detection neurons apoptosis inside the brain by magnetic resonance 1H-MRS.Methods Male SD rats of clean grade 20, make it cut off,which its two-side facial nerve trunk at stylomastoid foramen in the stretch of about 3mm, and the rats were evaluated for facial paralysis.Results On the injured side of 1 to 3week group there was complete facial nerve paralysis Both two sides of facial nucleus.Conclusion SD rat’s two sides of facial nerve high level injured model may be induced to two sides of facial nerve complete paralysis, and it’s very reproducible so it can provided experimental basis for detection neurons apoptosis inside the brain by magnetic resonance 1H-MRS.Objective To investigate the value of proton magnetic resonance spectroscopy (1H-MRS) technique for high level facial nerve injured in rats after two side of facial nucleus’s apoptosis observed in vivo feasibility.Methods 24 SD rats were selected, and their were divided into normal control group and model group.1H-MRS data obtained from 24 SD rats in GE 3.0T MR scanner were retrospectively analyzed. By using Functool 4.5.5 quantification of N-acetyl aspartate (NAA), creatine (Cr), choline (Cho) were obtained.Results Model group, NAA, NAA/Cr, NAA/Cho were lower than the normal control group, there were statistically significant differences,P<0.05(Model group and normal control group NAA were 7840.0±2671.46、10173.18±1500.33, t=3.159, P=0.003; Model group and normal control group, NAA/Cr were 0.94±0.33,1.27±0.14,t=3.782, P=0.001; Model group and normal control group NAA/Cho were 1.46±0.39,1.76±0.45, t=2.076, P=0.046). Model group’s Cho、Cho/Cr and normal control group were not statistically significant different, P>0.05(Model group and normal control group Cho was 5514.50±1801.51,6035.76±357.42,t=0.962, P= 0.343; Model group and normal control group Cho/Cr were 1.46±0.39,1.76±0.45, t=1.418,P=0.165).Conclusion The SD rat’s two sides of facial nerve high level cut off neurons of facial nucleus can lead to the facial nucleus of apoptotic neurons, and’ H-MRS technique can be used in vivo detection of the inner surface of the facial nucleus of apoptotic neurons.更多还原