【作者】 金砚淑；

【导师】 李江；

【作者基本信息】 吉林大学 ， 口腔医学， 2011， 硕士

【摘要】 目的:信号传导和转录激活因子(signal transducer and activators of transcription,STAT)是一种能够介导多种细胞因子和生长因子信号向细胞核内转录的因子,其能影响靶基因的转录,也可调节细胞的功能。STAT3是STAT蛋白家族中重要成员之一,在人类许多种实体肿瘤中表现为高水平的激活状态,与肿瘤细胞的抗凋亡,增殖和血管生成关系密切,已被确定为癌基因。活化的信号转导子和转录激活子3的蛋白抑制剂(Protein inhibitor of activated signal transducer and activator of transcription 3,PIAS3)为STAT3的特异性蛋白抑制剂,其通过与活化的STAT3结合进而阻止后者与DNA结合,因而抑制STAT3的转录激活活性。有研究显示,PIAS3在人体的前列腺、脑、乳腺、肾、肺、食道、皮肤、肝脏、胃及结直肠的恶性肿瘤中都有不同程度的高表达,提示PIAS3可能在肿瘤的发生、发展的过程中起到作用。涎腺肿瘤为口腔颌面部常见的肿瘤之一,STAT3和PIAS3在涎腺肿瘤中的表达情况国内外还少见报道,因此本课题通过免疫组织化学二步法研究STAT3和PIAS3在涎腺肿瘤中的表达情况以及与STAT3和PIAS3与涎腺肿瘤恶性程度的关系。方法:采用免疫组织化学二步法检测60例涎腺肿瘤(良性涎腺肿瘤30例:包括15例多形性腺瘤,15例基底细胞腺瘤;恶性涎腺肿瘤30例:包括15例黏液表皮样癌,15例腺样囊性癌)及30例正常涎腺组织中STAT3和PIAS3的表达情况;利用自动图像分析仪定量分析STAT3和PIAS3的相对含量(灰度)。结果:1.STAT3在涎腺肿瘤及正常涎腺中的表达:STAT3在恶性肿瘤组的表达水平显著高于良性肿瘤组,两组之间有显著性差异(p<0.05);STAT3在涎腺良性肿瘤组的表达情况高于正常涎腺组,两组的表达水平亦有显著性差异(p<0.05)。2.PIAS3在涎腺肿瘤及正常涎腺中的表达:PIAS3在涎腺恶性肿瘤组的表达水平明显高于良性肿瘤组,两组之间的差异显著(p<0.05);涎腺良性肿瘤组中PIAS3的表达高于正常涎腺组,两组表达水平亦有显著性差异(p<0.05)。3.STAT3及PIAS3表达的相关性:STAT3及PIAS3在正常涎腺组织、涎腺良性肿瘤组及涎腺恶性肿瘤组中的表达呈正相关(P<0.05)。结论:STAT3及其抑制因子PIAS3在涎腺肿瘤中的表达随着涎腺肿瘤恶性程度的提高而增强,提示了STAT3及PIAS3可能影响着涎腺肿瘤的发生、发展,并且与涎腺肿瘤的恶性程度密切相关,可能成为一条治疗涎腺肿瘤的新途径。更多还原

【Abstract】 Objective:STAT(signal of transducer and activators transcription) is a factor which can mediate the signal of cell factors and growth factors into nuclear. It can not only affect the transcription of target genes, but also regulate the function of cell. One of the important members of STAT is STAT3, which showed a high level of activation status in many types of human tumors.PIAS3 (Protein inhibitor of activated signal transducer and activator of transcription 3) is an inhibitory factor of STAT3, which can stop the combination of pSTAT3 and DNA. The researches showed that PIAS3 widely distributed in human malignant tumors.Salivary gland tumor is a kind of universal oral tumor, but the expressions of STAT3 and PIAS3 are rarely reported. This research is to study the expression of STAT3 and PIAS3 in salivary gland tumor.Methods:The expression of STAT3 and PIAS3 in salivary gland tumor and normal salivary gland tissues were detected by immunohistochemical assay,the automatic image analyzer was used to analyze the gray scale of the STAT3 and PIAS3.Results:(1)The positive rate of expression of STAT3 was significantly higher (p<0.05) in the group of malignant salivary gland tumor than in the group of benign salivary gland tumor,while the group of benign salivary gland was significantly higher (p<0.05) than that in normal salivary tissue.(2)The positive rate of expression of PIAS3 was significantly higher (p<0.05) in the group of malignant salivary gland tumor than in the group of benign salivary gland tumor,while the group of benign salivary gland was significantly higher (p<0.05) than that in normal salivary tissue.(3)The expression of STAT3 was positively correlated with the expression of PIAS3 in the salivary gland tumor (p<0.05) .Conclusion:The expressions of STAT3 and PIAS3 are closely related to the degree of malignance in salivary gland tumor. STAT3 and PIAS3 play an important role in the pathogenesis and development of salivary gland tumor, from which we could find a new way to treat salivary gland tumor in the further work.更多还原