【作者】 于令媛；

【导师】 许永玉；

【作者基本信息】 山东农业大学 ， 农业昆虫与害虫防治， 2011， 硕士

【摘要】 1大草蛉滞育和非滞育预蛹耐寒性测定实验室内测定了大草蛉滞育和非滞育预蛹的耐寒性指标。结果表明,大草蛉为短光照滞育型昆虫,在短光照(L : D=9 : 15)条件下,发育成滞育预蛹的预蛹重比长光照(L : D=15 : 9)下非滞育预蛹的预蛹重量低4.43mg,过冷却点(SCP)和结冰点(FP)(?13.31℃,?6.63℃)显著低于非滞育预蛹(?9.61℃,?3.98℃)。同非滞育预蛹相比滞育预蛹体内的含水量比率降低,分别为61.33%和63.00%。滞育预蛹体内脂肪占干重比率显著高于非滞育预蛹体内脂肪比率,体内蛋白质和可溶性糖含量均低于非滞育预蛹。分别测定滞育、非滞育预蛹在不同聚集度下的呼吸速率(Rr)和能量代谢速率(Rm)。滞育和非滞育预蛹的Rr和Rm均随群体聚集度的增加而降低,聚集度为Ⅰ和Ⅴ的处理间差异均显著。在不同聚集度下非滞育预蛹的Rr (132.53×10-3μL·g-1·s-1)和Rm(32.47×10-4 W/g)均显著大于滞育预蛹的Rr(60.31×10-3μL·g-1·s-1)与Rm(14.77×10-4 W/g)。2大草蛉预蛹抗寒能力的季节性变化2009年9月至2010年7月连续测定大草蛉自然种群预蛹的蛹重、过冷却点(SCP)和结冰点(FP)、体内含水量、脂肪含量、蛋白质和可溶性糖含量。大草蛉预蛹的SCP和FP在越冬中期(2010年1月)达到最低值为?17.53℃和?7.14℃,在夏季(2010年7月)达到最高值为?8.21℃和?3.65℃,越冬前9、10月SCP和FP低于7月但是差异不显著,显著低于越冬后4、5月份,总体呈现先增后减的趋势,与环境温度变化一致。除体内脂肪含量外,其他各项耐寒性指标变化趋势与之相同。脂肪含量在越冬初期即达到最高为46.81%,越冬期间逐渐减少,但仍高于其他时期,夏季7月降到最低为31.07 %。大草蛉耐寒性各指标的变化趋势与该虫所处月平均气温变化一致,越冬预蛹的耐寒性显著高于夏季预蛹和越冬后预蛹,耐寒能力具有明显的季节性变化。3大草蛉预蛹的滞育与耐寒性关系研究分别测定滞育和非滞育种群经过10℃低温驯化和未经过低温驯化预蛹的耐寒性。实验种群的非滞育预蛹经过低温驯化之后SCP降为?12.18℃,经过短光照诱导的滞育种群其SCP为?13.09℃,虽然稍低于前者,但两者差异并不显著。经过10℃低温驯化的滞育预蛹的SCP降到?14.97℃,同未经过低温驯化的滞育预蛹相比,差异显著。22℃短光照下饲养的预蛹,其SCP明显低于非滞育未经过低温驯化预蛹的SCP(?9.61℃),与非滞育个体经过低温驯化后的SCP(?12.18℃)无明显差异。滞育和低温驯化也对预蛹重、体内水分、脂肪、蛋白质和可溶性糖含量产生影响,短光照滞育和低温诱导后,可降低虫体预蛹重、水份、蛋白质和可溶性糖含量,增加脂肪含量。4大草蛉实验种群年龄-龄期两性生命表在实验室内22℃、15L ? 9D、RH80%条件下,饲养观察大草蛉种群,记录各龄期发育时间、存活数、死亡数、繁殖力等生活史数据,采用年龄―龄期两性生命表进行分析(Chi and Liu,1985;Chi,1988)。大草蛉卵期为45 d,幼虫孵化率达100%,成虫羽化率90.70%,其中51.28%为雄虫,48.72%为雌虫。成虫之前存活率为75%。新产的卵存活到雄成虫的几率为0.38,雌成虫为0.35。雄虫平均寿命为33.7 d,雌虫为32.4 d。单雌产卵总量最高为1289粒,单日总产卵量最高为104粒,产卵高峰期出现在第42 d。大草蛉的内禀增长率(r)为0.1258,周限增长率(λ)为1.134d–1,净生殖率(R0)为241.42后代/个体,种群世代平均周期(T)为43.63 d。新产的卵的期望寿命为48.85 d,雌成虫最大的再生产价值出现在第37天,同总产卵前期(34.29 d)一致。当种群出生率为0.1396、存活率为0.9944和死亡率为0.0056时将达到稳定。更多还原

【Abstract】 1. Cold hardiness of diapausing prepupa and non-diapausing prepupa of Chrysopa pallens (Rambur)In the laboratory, the cold hardiness ability of diapausing and non-diapausing prepupa of Ch. pallens was examined. The results showed that the diapausing prepupa of the lacewing was induced by the short-day photoperiods. In the condition of shorter photoperiod (L: D=9: 15), the supercooling point (SCP) (?13.31℃) and freezing point (FP) (?6.63℃) were lower than that in the condition of longer photoperiod (L: D=15: 9), (?9.61℃and ?3.98℃, respectively), and the differences were significant. Water content of diapausing pepupa was lower than non-diapausing prepupa (61.33% and 63.00%, respectively), the content of protein and soluble sugar in the body of diapausing prepupa were lower, too. But the fat content in the body of diapausing prepupa was higher than that in non-diapausing prepupa. The rates of respiration (Rr) and the rates of energy metabolism (Rm) of Ch. pallens with five different aggregation degrees were calculated. The five different aggregation degrees were represented by five different group sizes (1, 5, 10, 20 and 50 individuals). The results indicated that there were differences in the values of Rr and Rm among the five different aggregation levels. The values of Rr and Rm had negative correlation relationships with aggregation degrees. The Rr and Rm in the body of diapausing prepupa (132.53×10-3μL·g-1·s-1 and 32.47×10-4 W/g) were lower than that in non-diapausing prepupa (60.31×10-3μL·g-1·s-1 and 14.77×10-4 W/g).2. Seasonal changes in prepupa cold hardiness of Chrysopa pallens (Rambur)The weight, supercooling point (SCP), the content of water, fat, sugar and protein of prepupa of Ch. pallens natural population were detected from September 2009 to July 2010. The SCP and FP of Ch. pallens prepupa were lowest in January (?17.53℃and ?7.14℃, respectively) while hightest in July (?8.21℃and ?3.65℃, respectively). The pre-winter prepupa had lower SCP and FP than the summer and post-winter prepupa. Except for the content of fat, the variation tendency of all the other cold hardiness indexes first increased and then decreased. Changes of the fat content were contrary. The fat content of pre-winter prepupa (46.81%) was significantly higher than those in other seasons, in January to lowest (31.07%). The pre-winter prepupa had higher tolerance than the summer and post-winter prepupa. The cold tolerance of the prepupa varied obviously with seasons.3 The relationship between diapause and cold hardiness of prepupa of Chrysopa pallens (Rambur)The cold hardiness of diapausing / non-diapausing prepupa populations with a cold acclimation at 10℃and without cold acclimation ware determined. Under laboratory conditions, the SCP of non-diapausing prepupa with cold acclimation was ?12.18℃, while the SCP of diapausing population with short photoperiod induction was ?13.09℃. There was not significant difference between the two SCPs. The SCP of the diapausing prepupa with 10℃cold acclimation was ?14.97℃, showing a significant difference compared with that without acclimation. The SCP of prepupa under short photoperiod at 22℃was significantly lower than that of non-diapausing prepupa populations (?9.61℃). Short photoperiods and low temperatures maybe cause the prepupa weight, the water content, fat, protein and soluble sugar in the body to drop and the fat to increase.4 Age-stage, two-sex life table of Chrysopa pallens (Rambur)The life history of the green lacewing, Ch. pallens, was studied at 22°C, 15L ? 9D, RH 80% in the laboratory. The data of life history, such as development period, survival, mortality, fecundity and so on were recorded. The raw data were analyzed based on the age-stage, two-sex life table in order to take both sexes and the variable developmental rate among individuals and between sexes into consideration. All the eggs hatched successfully within 4-5 days, the preadult survival rate was 75%, 90.70% of adults emerged with 51.28% males and 48.72% females. The probability that a newborn egg survived to the adult stage was 0.38 for males and 0.35 for females. The mean longevity of male and female was 33.7 and 32.4 days, respectively. Maximum fecundity of each individual was 1289 eggs, maximum daily fecundity of each individual was 104 eggs, spawning peaked in the first 42 days.The intrinsic rate of increase (r), the finite rate of increase (λ), the net reproduction rate (R0) and the mean generation time (T) of Ch. pallens were 0.1258 d–1, 1.134 d–1, 241.42 offspring and 43.63 d, respectively. The life expectancy of a newborn egg was 48.85 days and the life expectancy of a newborn egg was 48.85 days. The maximum reproductive value of females was on the 37th day, which closed the total pre-oviposition period counted from birth (34.29 d). When the birth rate was 0.1396, survival rate was 0.9944 and death rate was 0.0056, the population of Ch. pallens was the most stable.更多还原