【作者】 许晓强；

【导师】 张巧花； 白林海；

【作者基本信息】 山西医科大学 ， 血液学， 2011， 硕士

【摘要】 目的：探讨弥漫大B细胞淋巴瘤(DLBCL)患者血清细胞因子白细胞介素(IL)-2、IL-6、IL-8、IL-10、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)表达水平与化疗耐药之间的相关性。方法：30例DLBCL化疗耐药患者为化疗耐药组,30例DLBCL化疗敏感患者为化疗敏感组,20例健康人作为健康对照组,ELISA法测定两组DLBCL患者治疗前、中、后及对照组血清中IL-2、IL-6、IL-8、IL-10、TNF-α、IFN-γ水平。结果：化疗耐药组患者血清中的IL-6、IL-10治疗前表达水平明显高于化疗敏感组患者治疗前和对照组表达水平(P值均<0.05),治疗缓解后明显低于治疗前(P=0.02、P=0.015)。DLBCL复发耐药时,患者血清中的IL-6、IL-10又高于缓解时水平(P=0.004、P<0.001)。化疗耐药组III-IV期患者治疗前IL-6水平明显高于Ⅰ-Ⅱ期患者(P<0.05)。其他细胞因子IL-2、IL-8、TNF-α、IFN-γ水平在DLBCL化疗耐药组、DLBCL化疗敏感组、对照组之间差异无统计学意义(P>0.05)。结论：DLBCL患者血清中的IL-6、IL-10表达水平的高低与DLBCL化疗疗效密切相关,可能与DLBCL细胞耐药有关。更多还原

【Abstract】 Objective:To investigate the correlation between expression levels of serum cytokines IL-2, IL-6, IL-8, IL-10, TNF-αand IFN-y in patients with diffuse large B-cell lymphoma(DLBCL) and chemotherapy resistance.Methods:30 cases of DLBCL patients with chemotherapy resistant,30 cases of DLBCL patients with chemotherapy sensitive and 20 cases of healthy individuals as normal control group were enrolled. The levels of serum cytokines IL-2, IL-6, IL-8, IL-10, TNF-αand IFN-y before, during and after treatment in both DLBCL groups and normal control group were detected by ELISA assay.Results:The expression level of serum IL-6, IL-10 before treatment in DLBCL in patients with chemotherapy resistance were significantly higher than that in DLBCL patients with chemotherapy sensitive and normal control group (P<0.05), however, that after treatment in DLBCL patients with chemotherapy resistance was significantly lower than that before treatment (P=0.02 and P=0.015). The level of serum IL-6, IL-10 in patients with DLBCL recurrence into drug resistance was higher than that during of remission (P=0.004 and P＜0.001). Before treatment, the expression level of serum IL-6 in patients withⅢ-Ⅳstage in chemotherapy resistant group was significantly higher than that in ones withⅠ-Ⅱstage (P<0.05). Levels of IL-2, IL-8, TNF-α, IFN-γin chemotherapy resistant group, chemotherapy sensitive group and the normal control group were no differences. (P>0.05).Conclusion:The expression levels of the serum IL-6, IL-10 were closely correlated with the chemotherapy efficacy of DLBCL, they may be involved in drug resistance of DLBCL更多还原