【作者】 孙平平；

【导师】 郭光伟；

【作者基本信息】 山西医科大学 ， 心胸外科学， 2011， 硕士

【摘要】 目的：基于连笔直写的生命体微结构血管支架采用了微纳米制造技术，由于构成材料分子大小尺度的变化，可能带来材料性能的改变，因此，必须对其进行全面的检测。我们将对其进行力学性能和生物相容性的检测。方法：我们首先通过缝合强度、爆破压力和径向顺应性对该血管支架进行力学性能检测；采用溶血率、体外动态凝血实验、血小板黏附实验对其进行血液相容性的检测；采用细胞培养MTT法、细胞形态学观察方法，研究该血管支架的细胞相容性。结果:该血管支架为类似于自然血管的三层结构，长2cm，内径6mm，内外层含有分布规则孔隙（大小10μm～30μm）的10%浓度的聚乳酸-乙醇酸（PLGA），中间层为无贯通孔隙的15%浓度的聚氨酯(PU)。该血管支架的径向顺应性达到4.03±0.56%/100mmHg，缝合强度为204.5±72.1N/cm2，爆破压力约为102±8KPa；该支架的溶血率为1.75%，小于ISO规定的5%，可认为这种支架无溶血作用；体外动态凝血实验中，该血管支架的抗凝血性能显著优于对照组载玻片（P＜0.05），而且扫描电镜观察血小板粘附较少，显示出该血管支架良好抗凝血性能；MTT比色法结果显示其细胞毒性为0～1级；细胞形态学观察显示L929细胞在该血管支架膜片的浸提液中呈梭形或三角形，贴壁良好。结论:基于连笔直写的生命体微结构成形技术制备的血管支架具有良好的机械力学性能、血液相容性和细胞相容性，满足组织工程血管支架的要求。更多还原

【Abstract】 Objective: To evaluate mechanical properties and biocompatibilities of the vascular scaffoldmanufactured with Micro-Tip Direct Writing technology.Methods: Mechanical properties of the vascular scaffold were measured by radial compliance,suture strength and bursting pressure separately. The blood compatibilities of the scaffold wereanalyzed by hemolytic rate test, in vitro dynamic blood clotting test, ant platelet adhesionexperiment. Cell compatibility was evaluated by MTT measurement and morphologicalobservation.Results: The scaffold showed radial compliance of4.03±0.56%/100mmHg, burst strength of102±8KPa, and suture retention strength of 204.5±72.1N/cm2. The hemolytic rate of the scaffoldwas 1.75% .It is lower than 5％which was ruled by ISO. The anti-coagulant property of thescaffolds was significantly better than that of glass slides in dynamic coagulation tests（P＜0.05).The anti-coagulant property of the scaffold was also confirmed by less platelet adhesion. MTTassay results showed that the cytotoxic grade of the novel polyurethane was 0~1. Cellmorphology observation showed that the L929 cells were spindle-shaped or triangular with goodstretch. Conclusion: The scaffold manufactured with the new technique processes goodmechanical properties, blood compatibility and cell compatibility.更多还原