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STZ处理树鼩胰岛β细胞再生及EGF、IGF-1表达的研究
Study on STZ Treatment to Tree Shrews Beta Cell Regeneration and EGF, IGF-1 Expression
【作者】 蒋婵；
【导师】 秦映芬；
【作者基本信息】 广西医科大学 ， 内分泌学， 2011， 硕士
【摘要】 目的:建立树鼩胰岛β细胞损伤模型,证实树鼩胰岛β细胞的再生及其与EGF、IGF-1表达的关系。方法:将适应饲养环境的32只成年树鼩随机分为两组,包括STZ处理组28只和正常对照组4只。2周内以STZ(每次120mg/kg)总计三次腹腔注射后再观察10天,根据血糖情况分为处理组A(血糖正常)5只,处理组B(血糖先升高后恢复正常) 8只,处理组C(血糖轻度升高但未达糖尿病标准)6只,处理组D(糖尿病组)5只。对照组4只,予以腹腔注射酸化生理盐水5ml/kg。3次注射STZ后3d、1w、10d检测血糖、称体重及实验前后空腹血胰岛素(FINS)、并计算胰岛素敏感指数;留取胰腺标本,光镜下观察胰岛病理形态学改变,应用HE染色观察胰岛炎症程度、以免疫组织化学方法检测胰岛素、胰岛组织中细胞再生相关因子Pdx-1、Ngn3、EGF、IGF-1的表达。结果:(1)A组、B组胰岛基本保持正常组织形态,细胞体积较正常,岛形较饱满,C组少量胰岛细胞胞浆内有空泡变性,D组胰岛β细胞数量明显减少,胰岛空虚。(2)与D组相比,B组树鼩胰岛表达INS显著升高(P<0.05),血清胰岛素水平上升,血糖水平下降。(3)STZ处理组的ISI均显著低于正常对照组(P<0.05);与D组比较,A、B、C组树鼩的ISI均显著升高(P<0.05),A、B组ISI显著高于C组,(P<0.05),但A、B组之间没有统计学差异(P>0.05)。(4)STZ处理组的PDX-1和Ngn3表达低于正常对照组(P<0.05);B组与C组、D组相比显著升高(P<0.05),但与A组没有统计学差异(P>0.05)。(5)STZ处理组的EGF和IGF-1表达低于正常对照组(P<0.05);B组与C组、D组相比显著升高(P<0.05),但与A组没有统计学差异(P>0.05)。结论:STZ处理后B组树鼩可能通过增加胰岛表达Pdx-1、Ngn3、EGF、IGF-1等相关因子,对实验性糖尿病树鼩残存β细胞的增殖和再生起到一定的促进作用,进而改善血糖代谢和胰岛素抵抗,并在一定程度上促进胰岛功能的恢复,降低血糖。
【Abstract】 Objective:Establishment of tree shrew model of pancreaticβcell injury, confirmed that the tree shrew isletβ-cells regeneration and the relationship with EGF、IGF-1 expression.Methods:Feeding the environment will adapt the 32 adult tree shrews were divided into two groups , including the 28 STZ treated group and control group 4.2 weeks to STZ(each 120mg/kg) injected a total of three times and then observed for 10 days,Were divided into treatment group A according to blood glucose( normal blood glucose)5,treatment group B(increased blood sugar returned to normal after the first)8,treatment group C(blood glucose increased slightly but less than diabetes standard)6,treatment group D(diabetic group) 5.E group,control group 4 .Control group received intraperitoneal injection of acidified saline 5ml/kg.STZ3 times after injection 3d,1w, 10d detection Blood glucose,weighed,Before the injection and death of insulin, and calculate Insulin sensitivity index. Pancreatic specimens collected,Observed under light microscope morphological changes of islet,HE staining of isletinflammation,Insulin was detected by immunohistochemistry, Measurement of islet Tissue cell regeneration of factor Pdx-1, Ngn3, EGF, IGF-1 expression.Results:(1)A group, B group remained normal islet morphology, cell volume compared to normal, relatively full island-shaped, C group had a small amount of the cytoplasm of islet degeneration, D group significantly reduced the number of pancreaticβcells, islet empty.(2)Compared with the D group, B group tree shrew islet Expression of INS was significantly higher (P <0.05),B group tree shrew increase in serum insulin levels, glucose levels decrease.(3)STZ treated group ISI was significantly lower than the control group (P <0.05); with the D group, A, B, C group tree shrew ISI were significantly higher (P <0.05), where A, B group and C group significant difference, ISI increased more significantly (P <0.05), A, B did not differ between groups (P> 0.05).(4)STZ treatment of PDX-1 and Ngn3 protein antigen lower than the control group (P <0.05); compared with C group, D group B group was significantly higher (P <0.05),There was no difference with the A group (P> 0.05).(5)STZ treatment of EGF and IGF-1 protein antigen lower than the control group (P <0.05); compared with C group, D group B group was significantly higher (P <0.05),There was no difference with the A group (P> 0.05).Conclution: STZ treated tree shrew islets may be expressed through the promotion of Pdx-1, Ngn3, EGF, IGF-1 and other related factors For the Tree shrew experimental Diabetes residualβ-cell proliferation and renewable play a role in promoting. Improve glucose metabolism and insulin resistance, and to some extent, promote the recovery of islet function and lower blood sugar.