【作者】 申金贵；

【导师】 叶品良；

【作者基本信息】 成都中医药大学 ， 方剂学， 2010， 硕士

【摘要】 目的：通过肺痨康(FLK)对实验性结核小鼠免疫指标的观察,研究肺痨康的免疫调节抗结核作用,为肺痨康治疗肺结核提供实验依据。方法动物造模后,随机分为FLK高剂量组、FLK中剂量组、FLK小剂量组、利福平组、异烟肼组、肺痨康异烟肼组、肺痨康利福平组,另设空白对照组,分别给予肺痨康、利福平、生理盐水灌胃28天,观察实验小鼠胸腺指数、T淋巴细胞亚群、IFN-γ、IgG改变情况及小鼠肝肺的病理学检测。结果：①FLK高剂量组能明显增加小鼠胸腺指数(P<0.05)；②FLK高剂量组CD4+T细胞数显著升高(P<0.01,P<0.05)；CD8+各组之间比较差异无显著性(P>0.05)；模型组CD4+/CD8+比值显著降低,其差异有显著性(P<0.01),FLK高剂量组明显减轻CD4+/CD8‘比值下降,其差异有显著性(P<0.01)；③FLK高剂量组增加小鼠结核模型血浆IFN-γ和IgG水平(P<0.05)。④与抗痨西药组比较,经病理学组织学观察,肺痨康加抗痨药组肝脏多发性坏死病变明显减轻。结论：①肺痨康治疗结核病具有免疫调节抗结核的作用。②肺痨康和西药组合用对结核小鼠模型的治疗有协同增效的作用；③肺痨康对抗痨化学疗法所致肝损害有一定的保护作用。更多还原

【Abstract】 Objective To study the effect of FeiLao-kang (FLK) on the changes of immunological indices of mouse tuberculosis model and provide the research evidence of FLK for the treatment of tuberculosis.Methods After the animal model establishment, the mice were randomly divided into the following groups:high-dose group of FLK, moderate-dose group of FLK, group of RFP, group of INH, group of FLK add RFP, group of FLK add INH and a control group. The RFP capsules, FLK, NS were given separately for 28 days. To observe the changes of thymus index, T-lymphocyte subsets, IFN-y, IgG in experiment mouse.Result①The high-dose group of FLK to a marked increase in thymus index of mice (P<0.05);②The number of CD4+ T cells increased significantly in high dose group of FLK (P<0.01, P<0.05); the number of CD8+ in each group Comparison between the difference was not significant (P> 0.05); The ratio of CD4+ /CD8+ were significantly in lower model group, the difference was significant (P<0.01), The ratio of CD4+ /CD8+ significantly reduce the decreased in high-dose group of FLK,the difference was significant (P<0.01);③the serum levels of IFN-y and IgG under the groups treated with high-dose FLK was increased (P<0.05).④To compare with the latter line antituberculosis drug group:to observe by the result of pathological histology, liver multiple thanatosis had lessened in FeiLao-kang group and the latter line antituberculosis drug group.Conclusion①FLK show definite immuno regulatory and anti-tuberculosis effects.②In the animal experiment, we can get good therapeutic efficacy from using FeiLao-kang group to treat TB.③FeiLao-kang had the certain protecting effect on liver pathologic lesion induced by antiphtisy and chemotherapy.更多还原