节点文献
柴胡止血液含药血清对置宫内节育器人子宫组织超氧化物歧化酶和钠钾ATP酶活力的影响
Observation on Chai-hu Hemostatic Oral Liquid to Activity of SOD and Na~+K~+-ATPase in Human Uterine Organism Inserted with IUD
【作者】 唐朋利；
【导师】 陈钢；
【作者基本信息】 成都中医药大学 ， 中医基础理论， 2010， 硕士
【摘要】 目的：研究调肝止血法治疗宫内节育器所致子宫出血副反应的作用机制,为进一步研究中医肝藏血理论提供实验依据,并为临床治疗血证提供新的思路和方法。方法：原代培养人子宫内膜组织,观察根据调肝止血法组方的柴胡止血液含药血清对培养的人子宫组织超氧化物岐化酶(SOD)和钠钾ATP酶活力的影响。采用黄嘌呤氧化酶法测定SOD的活力；采用无机磷定量法测定ATP酶的活力；采用考马斯亮兰蛋白测定试剂盒测定蛋白含量。结果：1.在未绝经有症状组中,与空白血清组比较,柴胡止血液含药血清组增强了SOD和钠钾ATP酶的活力(P<0.05)。2.在绝经组中,空白血清组与柴胡止血液血清组之间没有显著的统计学差异(P>0.05)。结论：调肝止血法可能通过增强子宫组织的SOD、钠钾ATP酶活力等作用机制,促进子宫组织的修复,从而达到治疗置宫内节育器所致子宫异常出血的目的。
【Abstract】 Purpose:To investigate the mechanism of Chaihu Zhixueye stopping bleeding to cure the uterine bleeding side-effect with IUD, in order to provide experimental foundation for further investigation of the basic theory of TCM such as liver storing blood, and to provide new thought and method to cure haemorrhagia.Method:we acquire human uterine organism by culture of organism and homogenization. Then observe the effect of the serum containing Chaihu Zhixueye by testing the activity of SOD and Na+K+-ATPase that the human uterine organism excrete.Results:Compare to the blank serum, serum of Chaihu Zhixueye can enhance the activity of SOD and Na+K+-ATPase (P<0.05).Conclusion:Serum of Chaihu Zhixueye can stop the bleeding to cure the side-effect of uterus with IUD through adjusting the founction of liver, that maybe due to serum of Chaihu Zhixueye can adjust the activity of SOD and Na+K+-ATPase that the human uterine organism excrete. So that serum of Chaihu Zhixueye can accelerate repair of the human uterine organism then stop the abnormal bleeding.