【作者】 张珞；

【导师】 曾倩；

【作者基本信息】 成都中医药大学 ， 中医妇科学， 2010， 硕士

【摘要】 [研究目标]通过对慢性盆腔炎患者疗前体液免疫和T淋巴细胞亚群的检测,研究慢性盆腔炎患者免疫状况,寻找其免疫紊乱规律,为进一步探索中医药调控盆腔局部环境、调整局部免疫功效奠定基础。[研究方法]本研究拟纳入成都中医药大学附属医院妇科住院部及门诊部2008年6月—2010年1月期间符合慢性盆腔炎湿热瘀结型诊断辨证标准病例117例,采用流式细胞术对其疗前外周血体液免疫和T淋巴细胞亚群进行检测,将所获得的数据资料使用Excel数据录入软件录入计算机,建立数据库。采用SPSS13.0统计软件对数据进行X2检验、频数分析、聚类分析、Logist回归分析等,得出统计结果。[研究结果]1.湿热瘀结型慢性盆腔炎患者免疫指标存在显著异常,其中以CD4+/CD8+值异常最为多见,共25例,占整个研究病例的21.37%。2.湿热瘀结型慢性盆腔炎患者中存在免疫异常者共71例,其免疫指标异常存在多种类型,以C3值伴C4值降低最为常见,共10例,占免疫异常者的14.08%。3.湿热瘀结型慢性盆腔炎患者的病程、孕产次数均与免疫指标异常呈正相关性；其中病程≤1年与病程>1年、孕产≤3次与孕产>3次免疫指标异常的差异有统计学意义(P<0.05)。4.湿热瘀结型慢性盆腔炎患者月经周期不同时期与免疫指标异常有相关性,以经期最易出现免疫指标失衡。5.湿热瘀结型慢性盆腔炎患者免疫指标异常与其年龄增加无相关性。(P>0.05)[结论]湿热瘀结型慢性盆腔炎患者存在免疫失衡现象；伴随病程的增加、孕产次数的增多免疫失衡机率增加,且经期较平时更易发生免疫失衡。更多还原

【Abstract】 ObjectiveThis study research the humoral immune and T lymphocyte of chronic pelvic inflammatory disease of damp-heat stasis pattern, to find the laws of their immune disorders, in order to further explore whether there is to improve Pelvic local environment, adjust the partial pelvic immune efficacy.MethodInto 117 cases of patients with standard of chronic pelvic inflammatory disease of damp-heat stasis pattern from June 2008 to January 2010 in Chengdu University of Traditional Chinese Medicine affiliated hospital outpatient and inpatient gynecological patients.Detect their humoral immune and T lymphocyte before treatment by Flow Cytometry(FCM).Data obtained will be entered computer,established database and collated by using Excel data.Then,they are dealed with SPSS13.0 statistical software for data X2 test, frequency analysis, cluster analysis, Logist regression analysis.Results1. There are significant immune differences exist among the patients who suffered from chronic pelvic inflammatory disease of damp-heat stasis pattern. The most common one is the difference of CD4+/CD8+ among all the others,which is the highest for 25 cases,accounting for21.37%.2. The totality of immune imbalance of chronic pelvic inflammatory disease with damp-heat stasis pattern is 71 cases.Their immune abnormalities are various types,and the frequency of C3 value and the value of C4 reduce is the highest for 10 cases, accounting for 14.08% of immune imbalance.3. The immune abnormalities of chronic pelvic inflammatory disease with damp-heat stasis pattern has positive correlation with the duration and motherhood times, there are significant differences among the patients with the duration more than one year, as well as the ones who had be pregnant for more than three times. (P<0.05)4. There is correlation between the different time of the menstruational cycle and the immune imbalance, that is immune imbalance appeared easily during the menstrual period.5. The immune abnormalities have no direct relevance with age increase. (P>0.05)ConclusionThere is phenomenon of immune imbalance among the patients who suffered from chronic pelvic inflammatory disease of damp-heat stasis pattern,which is increasing with the duration and motherhood times and the most obvious in menstrual performance.更多还原