【作者】 胡金鹿；

【导师】 陈伟平；

【作者基本信息】 浙江大学 ， 营养与食品卫生学， 2011， 硕士

【摘要】 目的：研究蚕蛹油对高脂血症大鼠载脂蛋白及脂代谢相关酶的影响,探讨其调血脂作用及机理。方法：1.采用微波萃取技术和尿素包合法得到的蚕蛹源不饱和脂肪酸比传统方法制取的蚕蛹油含有的不饱和脂肪酸如α-亚麻酸,亚油酸等含量均高,而饱和脂肪酸含量很低。2.动物实验：60只雄性SD大鼠,按体重并参考血脂水平随机分为6组：正常对照组,高脂血症模型组,蚕蛹油高(7.5ml/kg)、中(6.0ml/kg)、低(4.5ml/kg)剂量三组,紫苏油阳性对照组(紫苏油6.0ml/kg)。适应期1W后分别喂养对应饲料,高脂造模成功后开始灌胃,每两周检测血液中甘油三酯(TG)、总胆固醇(TCH)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、载脂蛋白A I(ApoAⅠ)、载脂蛋白B (ApoB)、卵磷脂胆固醇转移酶(LCAT)、脂蛋白脂酶(LPL)、肝脂酶(HL)水平,第六周处死后,称量肝重并检测肝中脂蛋白脂酶(LPL)、肝脂酶(HL)水平。结果：①给予蚕蛹油治疗后,可明显降低大鼠血清TC、TG、LDL-C、ApoB和提高HDL-C、ApoAI、HL、LPL、LCAT水平。②载脂蛋白及脂代谢酶的改变早于血脂和血脂蛋白的改变。③蚕蛹油可以剂量依赖性地降低大鼠血清中TG的水平。④与阳性(紫苏油)对照组相比,蚕蛹油组降低血脂的效果优于紫苏油组。结论：蚕蛹油可以通过激活载脂蛋白和脂代谢相关酶,调整血清中血脂和脂蛋白的含量,从而达到治疗高脂血症的目的。更多还原

【Abstract】 ObjectiveTo research the effect of silkworm chrysalis oil on apoprotein and lipid-metabolize-enzyme level of hyperlipidemia rats,and investigate the mechanism of how to regulate the blood lipids.Methods1. Silkworm chrysalis oil was extracted by combination of microwave extraction technology and urea adduction fractionation. Silkworm chrysalis oil riches in unsaturated fatty acids such as a-linolenic acid, linoleic acid. And the levels of unsaturated fatty acids were higher than pupa oil which was extracted by traditional methods.2. A total of 60 male SD rats were equally randomized into 6 group by weight: normal control group, high-fat control group, low,medium and high dose of silkworm chrysalis oil group, purple perilla seed oil positive control group, and feeding them with corresponded feedstuff., after building a high-lipid model, observing TG, TCH, HDL,LDL, ApoA I, ApoB, LCAT, HL, LPL level in the blood every two week, then killing the rats in the sixth week, observing LPL, HL level in the liver.Results ①After giving silkworm chrysalis oil treatment, it can significantly reduce serum TC, TG, LDL-C, ApoB and increase HDL-C, ApoAI, HL, LPL, LCAT levels in the rats.②The changes of apolipoprotein and lipid metabolic enzyme are earlier than the lipid and lipoprotein in the rats.③Silkworm chrysalis oil can reduce the dose of serum TG level and also have dose dependence.④Conpared with the positive (purple perilla) control group,the silkworm chrysalis oil is better in control the lipid level.ConclusionThe silkworm chrysalis oil can regulate the plasma lipids and lipoprotein level in the blood by activating apoprotein and correlated lipid-metabolized-enzyme, thereby to treat the hyperlipidemia.更多还原