一株番荔枝内生真菌F-31的化学成分研究

【作者】 葛瀚麟；

【导师】 戴均贵； 邹建华； 杨金玲；

【作者基本信息】 北京协和医学院 ， 生药学， 2010， 硕士

【摘要】 植物内生真菌是天然活性化合物的一个重要来源。本论文对一株来源于海南刺果番荔枝、能够抑制肿瘤细胞增殖的内生真菌(F-31)进行了菌种鉴定、放大发酵培养以及活性化学成分的研究。菌种鉴定表明,菌株F-31可能为黑团孢霉属的一个新种(Periconia sp.)。应用硅胶柱色谱、薄层色谱、Sephadex LH-20凝胶柱色谱、正相及反相高效液相色谱等各种色谱手段对F-31发酵液的乙酸乙酯提取物和菌丝体的水层提取物分别进行了分离纯化,共得到11个化合物,并采用各种现代波谱技术对分离到的化合物进行了结构鉴定,其中6个化合物为新化合物。这些化合物包括：3个倍半萜类化合物(4S,5R,9S, 11S,12S)-Periconone A (1)、(3S,6S,7R,8S)-Periconone B (5)和Chloranthalactone E (6),1个二萜类化合物(6S,9R,10R,1 2R,16R)-Periconone C (3),1个单萜内酯(-)-(1R,4S,6R,7R)-2-Caren-4,9-olide (4),1个内酯类化合物4-(3’-Hydroxybutyl)-4H-furan-1-one (11),2个氨基酸类化合物L-色氨酸(8)和L-苯丙氨酸(10),1个醌类化合物7-hydroxy-11β,12α-di methyl-9,10-dihydronaphthalene-1,6-dione (2),1个香豆素类化合物5,7-二甲氧基-6-羟基香豆素(7),1个腺苷类化合物1,2,4-triazole-(1’R,2’R,3’R,4’R)-nucleosi des (9).上述化合物中,化合物1和3为两个新骨架化合物。体外评价结果显示：上述化合物对HCT-8、Bel-7402、BGC-823、A549、A2780、MCF-7六株肿瘤细胞的增殖均无明显抑制作用。更多还原

【Abstract】 Plant endophytic fungus is an important source of natural active compounds. An endophytic fungus F-31 (identified as Periconia sp.) derived from Annona muricata L which can inhibit proliferation of several tumor cells was selected for the chemical investigation.Six new compounds, (4S,5R,9S,11S,12S)-Periconone A (1), 7-Hydroxy-11β,12α-dimethyl-9,10-dihydronaphthalene-1,6-dione (2), (6S,9R,10R, 12R,16 R)-Periconone C (3), (-)-(1R,4S,6R,7R)-2-Caren-4,9-olide (4), (3S,6S, 7R,8S)-Periconone B (5),4-(3’-Hydroxybutyl)-4H-furan-l-one (11), along with five known compounds, Chloranthalactone E (6),5,7-Dimethyl-6-hydroxycoumarin (7), L-Tryptophan (8), 1,2,4-Triazole-(1’R,2’R,3’R,4’R)-nucleosides (9), L-Phenylalanine (10), were isolated from the ethyl-acetate extraction of broth and water-soluble fraction of mycelia by means of chromatographic techniques and identified by various spectroscopic methods.The in vitro pharmalogical evaluation results displayed that the above compounds exhibited no inhibitory effects on the proliferation of six tumor cell lines (HCT-8, Bel-7402, BGC-823, A549, A2780 and MCF-7).更多还原