【作者】 崔艳；

【导师】 苏云明； 关利新；

【作者基本信息】 黑龙江中医药大学 ， 药理学， 2010， 硕士

【摘要】 目的:研究西洋参茎叶皂苷(PQS)对大鼠局灶性脑缺血损伤的神经保护作用及作用机制。方法:采用雄性Wistar大鼠,PQS 50、100㎎·㎏-1灌胃给药,连续7d后利用线栓法建立大鼠大脑中动脉栓塞模型,造模后0h、24h按Longa法进行2次神经功能评分,光镜观察脑组织病理形态改变;并采用酶联免疫吸附法(ELISA法)检测造模24h后患侧脑组织中NGF及TGF-β1的含量。结果:1.PQS能明显改善局灶性脑缺血大鼠神经功能缺损体征,降低神经功能缺损评分,减轻缺血脑组织病理形态学改变。2. PQS能明显提高局灶性脑缺血大鼠脑组织NGF的含量。3. PQS能明显提高局灶性脑缺血大鼠脑组织TGF-β1的含量。结论:PQS对局灶性脑缺血损伤具有保护作用,其作用机制可能与诱导缺血脑组织中NGF、TGF-β1的表达有关。更多还原

【Abstract】 Objective: To investigate the protective effects of Panax quinquefolium saponins from steams and leaves (PQS) on focal cerebral ischemia damage and its mechamism.Method:The male rats were pretreated with PQS 50 and 100㎎·㎏ -1 ig, one time per day for 7d, and then subjected to focal cerebral ischemia damage induced by a middle cerebral artery occlusion(MCAO) for 24h. Determined neurological symptom by Longa’s score at the point 0h,24h after cerebral ischemia, and then drawn, brain tissue pathological changes was observed by light microscope; and using enzyme-linked immunosorbent assay (ELISA) test to detect the contents of NGF and TGF-β1 seperately in ipsilateral brain tissue.Results:1.PQS can significantly improve the focal cerebral ischemia in rats signs of neurological deficit, reduced neurological deficit scores, reduce the pathological changes of ischemic brain tissue.2.PQS can significantly increased the content of NGF in rat brain after focal cerebral ischemia.3.PQS can significantly increased the content of TGF-β1 in rat brain after focal cerebral ischemia.Conclusions: PQS has protective effects on focal cerebral ischemia damage and its mechanism may be related to inducing the expression of NGF and TGF-β1.更多还原