【作者】 朱志中；

【导师】 唐强；

【作者基本信息】 黑龙江中医药大学 ， 康复医学与理疗学， 2010， 硕士

【摘要】 目的:观察针刺与康复训练相结合对环磷酰胺免疫抑制大鼠模型脾脏指数、血清IL-2、IL-4及T细胞亚群的影响。方法:将40只健康SD大鼠(雄性)体重200-250g。完全随机分为5组:正常组、模型组、康复治疗组、针康组、针刺治疗组,每组8只。除正常组和模型组外,其他各组分别给予针刺或康复治疗。每日1次,治疗10天后,取大鼠脾脏及血清。计算脾脏指数,检测外周血CD4~+T细胞和CD8~+T细胞的含量。用放射免疫法检测血清中IL-2和IL-4含量。结果:康复组,针康组大鼠的脾脏指数与模型组比较有非常显著性差异(P<0.01),针刺组与模型组比较有显著性差异(P<0.05),与针刺比较康复训练对提高大鼠脾脏指数疗效更好,两者结合效果优于单纯针刺;模型组IL-2水平明显低于正常组,康复组,针刺组,针康组与模型组比较均有非常显著性差异(P<0.01);说明针刺和康复训练均能明显提高免疫抑制大鼠底下的IL-2水平;模型组血清IL-4水平显著升高,与正常组比较有显著性差异(P<0.05)。康复治疗和针康治疗都可降低免疫抑制大鼠血清IL-4水平,与模型组比较具有统计学意义(P<0.05);模型组大鼠CD4+T细胞水平明显下降,与正常组比较有显著性差异(P<0.05);经针刺和康复治疗后,针刺组和针康组大鼠外周血CD4~+T细胞水平显著升高,与模型组比较有非常显著性差异(P<0.01)。实验各组CD8~+T细胞水平于治疗前后无明显变化(P>0.05)。结论:针刺结合康复训练能提高免疫抑制大鼠脾脏指数;提高免疫抑制大鼠血清IL-2含量,降低大鼠血清中IL-4的含量;针刺结合康复训练能够显著提高免疫抑制大鼠低下的CD4~+T细胞水平,提高机体的细胞免疫功能。更多还原

【Abstract】 Objective: To observe the impact of acupunture combined rehaibilitation to spleen index,IL-2、IL-4、T lymphocyte subsets of immunosuppressive rat model.Method: To divided 40 healthy SD rats (male,weight 200-250g) into 5 groups randomly : normal group (NG) , model group(MG) , acupunture group(AG) , rehabilatation group(RG) , acupunture combine rehabilitation group(ARG),each with 8 rats. Treat each group by acupunture or rehabilitation except the model group,once per day,10 days later,took the spleen and the blood serum of the rats. Caculate the spleen index. Use radioimmunoassay and malondialdehydein(MDA) technique to test the contents of IL-2、IL-4 of each group. Test contents of T lymphocyte subsets.Result: Compared with the MG,the RG and ARG can increase the spleen index of rats with great significant difference (P<0.01) ,The AG can increase the spleen index of rats with significant difference (P<0.05). Compared with the AG,the RG work better in spleen index,acupunture combine rehabilitation work much better;Compared with the MG,the RG、AG and ARG can improve the contents of IL-2 of rats with great significant difference (P<0.01);The RG and ARG can decrease the contents of IL-4 of immunosuppressive rat with significant difference (P<0.05);After the treatment the level of CD4+TC of AG and RAG significantly increased than MG(P<0.01).Conclusion: Acupunture combine rehaibilitation treatment can increase the spleen index of immunosuppressive rats. This treatment also can increase the contents of IL-2,decrease the contents of IL-4.Improve the level of CD4+TC. Acupunture combine rehaibilitation work much better.更多还原