【作者】 叶忠伟；

【导师】 郑小伟；

【作者基本信息】 浙江中医药大学 ， 中医学， 2010， 硕士

【摘要】 目的：从加味当归补血汤对高脂血症大鼠肾组织结缔组织生长因子(CTGF)蛋白及其mRNA表达影响的角度,探讨其抗肾损害的作用机制。方法：用加味当归补血汤对高脂饲料喂养法致大鼠肾损害模型进行干预,实验大鼠随机分为正常组、模型组、舒降之组、加味当归补血汤低、中、高剂量组,各用药组连续干预给药12周后,用免疫组织化学技术半定量检测CTGF蛋白的表达水平,荧光定量逆转录聚合酶链式反应法(RT-PCR)检测CTGFmRNA的表达水平,从各组别的CTGF蛋白及其mRNA的表达水平探讨高脂血症致肾损害及加味当归补血汤对其的作用机制。结果：免疫组织化学检测结果显示模型组肾组织CTGF蛋白表达明显高于正常组(P<0.01),各治疗组大鼠CTGF蛋白的表达均有不同程度的减少,以加味当归补血汤高剂量组最明显,其次分别为阳性对照组、中药中剂量组、中药低剂量组,荧光定量逆转录聚合酶链式反应法(RT-PCR)检测结果显示模型组肾组织CTGFmRNA表达明显高于正常组(P<0.01),各治疗组大鼠CTGFmRNA的表达均有不同程度的减少,以加味当归补血汤高剂量组最明显,其次分别为阳性对照组、中药中剂量组、中药低剂量组。结论：加味当归补血汤对高脂血症大鼠肾损害的防治机制可能与下调CTGF蛋白及mRNA的表达有关。更多还原

【Abstract】 Jiawei Dangguibuxue Tang Has an Impact on the Expression of Renal Tissue in Rats of Hyperlipidemia CTGF Protein and mRNAObjective:From the Jiawei Dangguibuxue Tang in hyperlipidemic rats with renal connective tissue growth factor (CTGF) protein and mRNA expression, explore its mechanism of action of anti-renal injury.Methods:With the Modified Dangguibuxue Tang pairs of high-fat diet for renal injury model of intervention, rats were randomly divided into normal group, model group, impastation group, flavored Dangguibuxue Tang low, middle and high-dose treatment group. The treatment groups after 12 weeks were administered continuously, using semi-quantitative immunohistochemical detection of CTGF protein expression, fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) detection of the expression level of CTGF mRNA. From the various groups of CTGF protein and mRNA expression levels explore hyperlipidemia-induced renal damage and Jiawei Dangguibuxue Tang its mechanism of action.Results:Immunohistochemistry results showed that the model group CTGF protein expression in renal tissue were significantly higher than the normal group (P<0.01), the treated rats the expression of CTGF protein in varying degrees of reduced,especially to Jiawei Dangguibuxue Tang high-dose group of the most obvious. Followed by the positive control group, the dose of Chinese medicine, Chinese medicine low dose group. Fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) tests showed that the model group kidney CTGF mRNA expression was significantly higher than the normal group (P<0.01), the treated rats CTGF mRNA expression of varying degrees of reduced, especially to Jiawei Dangguibuxue Tang high-dose group of the most obvious. Followed by the positive control group, the middle-dose group, the low-ose group.Conclusions:Jiawei Dangguibuxue Tang on Hyperlipidemia prevention and treatment of renal injury in rats may be related to reduced CTGF protein and mRNA expression.更多还原