【作者】 岳兰；

【导师】 谭继英；

【作者基本信息】 兰州大学 ， 医学免疫学， 2010， 硕士

【摘要】 目的：检测Jagged-1蛋白在宫颈鳞状上皮细胞癌(Squamous cell carcinomasSCC)、宫颈腺癌和卵巢浆液性囊腺癌组织中的表达,探讨Jagged-1蛋白在宫颈癌和卵巢癌发生发展中的作用机制。方法：采用免疫组织化学EnVision法,对30例宫颈SCC、15例宫颈腺癌、14例宫颈正常组织及44例卵巢浆液性囊腺癌、14例正常卵巢组织中Jagged-1蛋白进行检测,用妊娠早期绒毛Jagged-1阳性表达作为阳性对照,用PBS代替一抗作为空白对照,根据肿瘤细胞染色程度以及阳性细胞所占百分比进行综合评分。采用SPSS13.0软件进行统计分析宫颈正常组织、宫颈鳞癌和腺癌中Jagged-1蛋白的表达,以及卵巢正常组织、卵巢浆液性囊腺癌组织中Jagged-1蛋白的表达水平,与病理组织学分级和临床分期等的关系。结果：1. Jagged-1蛋白在宫颈癌中的表达：Jagged-1蛋白在宫颈癌中主要表达在癌细胞的细胞核,在宫颈正常组织中阳性着色定位在基底细胞,其阳性表达为黄色或棕黄色颗粒,其在宫颈鳞癌、腺癌、正常组织中阳性表达率分别为66.7%、80.0%、21.4%。鳞癌、腺癌中Jagged-1蛋白的表达均高于正常组织,差异有统计学意义(P<0.05),鳞癌和腺癌相比差异无统计学意义(P>0.05)。2. Jagged-1蛋白在卵巢癌中的表达：Jagged-1蛋白在卵巢浆液性囊腺癌中表达在癌细胞的细胞浆和细胞核,在卵巢正常组织中阳性定位在部分颗粒细胞。其阳性表达亦为黄色或棕黄色颗粒,在卵巢浆液性囊腺癌中及正常组织中的阳性表达率分别为68.2%和28.6%,差异有统计学意义(P<0.05),在卵巢癌中Jagged-1蛋白的表达与卵巢癌患者年龄、肿瘤大小、临床分期无相关性(P>0.05),但随着卵巢癌病理分级增高,Jagged-1蛋白的阳性表达率增高,在病理2、3级中Jagged-1蛋白阳性表达率79.3%,明显高于1级阳性率(46.4%),G2、G3级与Gl级相比,P<0.05,差异有统计学意义。结论：1. Jagged-1蛋白在宫颈SCC和宫颈腺癌组织中均有较高的阳性表达率。2. Jagged-1蛋白在卵巢浆液性囊腺癌组织中高表达,且病理组织分级越高,Jagged-1阳性表达率越高,提示随着病理分级的增高,其表达上调。更多还原

【Abstract】 ObjectiveTo study on the expression of Jagged-1 protein in cervical cancer and ovarian serous cystadenocarcinoma, so as to explore Jagged-1 relationship in the mechanism about cervical cancer and ovarian serous cystadenocarcinoma.MethodEn Vision immunohistochemical (IHC) technique was used to assay the expression of Jagged-1 protein in 30 cases of cervical squamous cell carcinomas,15 cases of cervical adenocarcinoma,14 cases of normal cervical tissue and 44 cases of ovarian serous cystadenocarcinoma,14 cases of normal ovarian tissue. The sample of villi in early trimester of pregnancy was used as positive control and PBS replace the first antibody as blank control. According to the staining degree and the percent of positive cell in tumor cell, we divide into glade to be evaluated. The recorded data were analyzed using SPSS software 13.0 version.Results1. The expression of Jagged-1 protein in cervical carcinoma:Jagged-1 protein positive in cervical carcinoma express in cell nucleus of tumor cells. But Jagged-1 protein was less expressed in basal cell in normal tissue of cervix. The positive rate of cervica squamous cell carcinomas, adenocarcinoma, and normal tissue are 66.7%,80.0% and 21.4%, respectively. The expression of Jagged-1 protein in cervical carcinoma was higher apparently than normal tissue. There were significant differences in them. (P<0.05)2. The expression of Jagged-1 protein in ovarian cancer:Jagged-1 protein was expressed in cytoplasm and nucleus, and was positive in granular cell in normal tissue of ovary. The expression rate of Jagged-1 protein in the serous cystadenocarcinoma, normal ovarian tissue were 68.2%,28.6%, respectively. There were significant differences in ovarian serous cystadenocarcinoma and normal ovarian tissue (P<0.05). In ovarian cancer group, there was no significant difference for Jagged-1 expression in different age patients groups. And there was no difference among different tumor size and clinical stages. But the percent of Jagged-1 protein enhanced according to pathological grade change. There were significant differences in pathological grade 2 and 3 (79.3%) to grade 1 (46.4%), P<0.05.Conclusions1. Jagged-1 protein is positive expressed in both cervical squamous cell carcinomas and adenocarcinoma.2. Jagged-1 protein is positive expressed highly cystadenoma in ovary. And the expression of Jagged-1 protein is more positive with the pathological grade change. It suggested Jagged-1 is related in the cervical carcinoma and ovarian serous cystadenocarcinoma.更多还原