【作者】 王菲；

【导师】 杨官品；

【作者基本信息】 中国海洋大学 ， 海洋生物， 2010， 硕士

【摘要】 本研究通过富集培养,从沈抚灌区石油污染土壤中分离到五株以菲、芘为惟一碳源和能源生长的细菌,定为PAHs高效降解菌。经过形态观察、生理生化实验分析鉴定分别为苍白杆菌(Ochrobactrum sp.)、荧光假单胞菌(Psedomonas fluorescens)、戈登氏菌(Gordona sp.)、芽孢杆菌(Bacillus sp.)和分枝杆菌(Mycobacterium sp.)。利用室内模拟方法,研究中、低浓度多环芳烃(PAHs)污染土壤的微生物修复效果,阐明了土壤微生物(接种和土著)与(PAHs)降解的关系,结果表明：投加PAHs高效降解菌可以促进土壤中PAHs的降解,2周内效果显著；典型PAHs降解的难易程度菲<蒽<芘<苯并(a)芘>蔰；细菌种群丰度和多样性均与PAHs降解呈负相关关系,同一处理细菌种群结构随时间变化不大。对于中、低浓度PAHs原位污染土壤,增强土著菌的活性是提高土壤PAHs降解率的有效途径之一。更多还原

【Abstract】 The microbial remediation of long-term sewage irrigated soil (PAHs,1.34 mg kg-1) in Shen-fu irrigation area was carried out into laboratory.The effective PAHs-degrading strains were isolated by enrichment culture from this oil-contaminated soil,which are capable of growing on pyrene and phenanthrene as sole carbon and energy source. The strains were identified as Ochrobactrum,Pseudomonas,Gordona, Bacillus and Mycobacterium respectively according to the results of morphology analysis, physio-biochemical tests. A laboratory simulatiom test was conducted to study the microbial remediation of soils contaminated by medium and low concentration polyeyelie aromatic hydrocarbons(PAHs), and the relationship between the degradation of PAHs and the inoculated and indigenous microbes. The addition of high-effective PAHs-degrading bacteria promoted the biodegradation of soil PAHs, and the effect is remarkable in the first two weeks. The biodegradation of test PAHs was phenanthrene<anthracene<yrene<benzo[a]pyrene<chrysene, and negatively correlated with the diversity/abundance of soil bacterial population.In the same treatments, soil bacterial population structure varied less with time, and hence, to increase the avtivity of indigenous microbes would be an effective way to remediate the farmland soils contaminated by medium and low concetration PAHs更多还原