【作者】 蔡中芳；

【导师】 赵长增； 陈佰鸿； 彭坚；

【作者基本信息】 甘肃农业大学 ， 果树学， 2010， 硕士

【摘要】 四季花龙眼具有周年开花结果的习性、坐果率高、带蜂蜜风味的肉质果实等优点。在很多方面优于常规品种,其树姿开张、树体矮化,适于高密度栽培种植,所以研究四季花龙眼的花芽分化的过程和影响因素,是合理调控花芽分化进程和提高栽培效益的基础。本试验以5-6a的四季花龙眼为试材,研究了冬梢花芽分化进程中碳素营养和相关生理活性的变化动态。采用石蜡切片法显微观察其花芽的形态分化,统计各分化时期的花芽分化率,测定其芽体和叶片的可溶性总糖、蔗糖、果糖、还原糖、淀粉和蛋白质含量及淀粉酶和过氧化物酶活性,分析了可溶性蛋白PAGE图谱、淀粉酶同工酶谱和过氧化物酶同工酶谱。从对比的角度探讨了不同器官营养物质变化与花芽分化的关系。结果表明：1、四季花龙眼花芽形态分化过程可划分为未分化时期、花序原基分化期、花序主轴分化期、多级侧花序快速分化期和花器官分化期。在整个花芽分化过程中,顶芽多处于休眠或花芽和叶芽的中间状态,不能作为花芽分化起始的标志,而基部腋芽从露红点开始,较顶芽率先进入花芽分化期,且各分化时期有明显的阶段性,所以花芽分化应以腋芽形态分化为准。2、在花芽分化期,叶片和芽体的各种可溶性糖含量的变化均呈现“低-高-低”的变化趋势,但芽体含量的变化幅度较大,其含量也高于叶片。叶片内淀粉含量变化呈显著的降低趋势而芽体内淀粉含量则呈上升趋势。与叶片相比较,芽体中的可溶性总糖、还原糖和蔗糖的含量变化与花芽分化的相关度更高,而芽体和叶片的淀粉含量与花芽分化率无显著相关性,说明芽体内较高的可溶性糖有利于花芽分化的进行,而淀粉积累水平并非成花的决定性因素。3、成花过程中,成熟叶片的蛋白质含量,α-淀粉酶(α-AMY)活性和过氧化物酶(POD)活性均高于嫩叶含量,并在成花期有明显的变化规律,说明这些因素在成花过程中起到一定的积极意义。可溶性蛋白的SDS-PAGE凝胶电泳分析呈现有固定蛋白条带220KD,80KD和55KD,在花序主轴分化期和花器官快速分化期出现较为清晰的特异蛋白条带30KD和25KD。α-AMY同工酶酶谱呈现出逐渐增亮的条带。POD同工酶酶谱在花芽分化初期和花序原基分化期出现了新的条带,可作为分化的起始标志。它们的酶表达量的差异引起了酶活性的变化,说明SDS-PAGE和PAGE凝胶电泳呈现的条带变化与四季花龙眼花芽分化进程有密切的关系。更多还原

【Abstract】 Sijihua longan has many advantages which are the habit of anniversary Blossoming, high fruit set, fleshy fruits with flavor of honey and so on. Its many aspects are superior to regular varieties, which include tree vigor Opening, tree stunting and the plant with high density. So to research the process and impact physiologic factors of flower bud differentiation is foundation of reasonable regulate flower bud differentiation process and improve the cultivation benefits.Five to six-year-old sijihua longan were used as the experimental materials to research the change of nutrition dynamic during flower bud differentiation in its winter shoots. The morphological differentiation process of the flower bud differentiation was observed by the paraffin cut section method, flower bud differentiation rate of every period was calculated, the contents changing of total soluble sugar, sucrose, fructose, reducing sugar and starch in buds and leaves were determined, and the contents of soluble protein and the activity changes of a-AMY and POD in matured leaves and young leaves were determined, and analyzed the graphs of soluble protein by SDS—PAGE electrophoresis, a-AMY isozyme and POD isozyme. The study, in the view of comparison theory, explores the relationship between flower buds differentiation and the change of nutrition dynamic in different organs. The analysis indicates that:1. Process of flower bud development can be divided into 5 stages which are non-differentiation, inflorescence primordial differentiation, main axle of inflorescence differentiation, multistage side inflorescence fast differentiation and colored organ differentiation. During the flower bud differentiation, apical bud was in intermediate states between hibernation or flower bud and leaf bud, which could not be a sign of beginning of flower bud differentiation, but at the beginning of red point expose, the base axillary bud was the first to get into flower bud differentiation than apical bud, and which had obvious staged in differentiation, so flower bud differentiation should subject to morphology differentiation of axillary bud.2. During the flower bud differentiation, the change trends of soluble sugar content in leaves and buds was "low-high-low", but the change width of content in buds was larger, and content was higher than leaves. The changing trend of starch content in leaves was reduced markedly, while in contrary for buds, which reflected the relationship of the buds and leaves was sink and source respectively during the flower bud differentiation. The correlation of the changing magnitude of total soluble sugar, sucrose and reducing sugar in buds and the flower bud differentiation process was higher compared with leaves, while the correlation of the starch content changes in buds and leaves and the flower bud differentiation process was no significance, which indicated the higher soluble sugar content in buds was to the benefit of flower bud differentiation, but the accumulation level of starch was not determining factor of flowering.3. During the flower bud differentiation, the protein content, the a-AMY activity and POD activity in matured leaves were higher than young leaves, which had obvious change during flowering period, which indicated those factors proved to be significant to flowering. There were fixed protein bands in protein analysis by SDS-PAGE electrophoresis, which were 220KD,80KD and 55KD, it appeared clearer specific protein bands in main axle of inflorescence differentiation and colored organ differentiation, which was 30KD and 25KD.a-AMY isozyme bands presented gradually brightening. POD isozyme presented new bands in beginning period and inflorescence primordial differentiation, which could be a sign of beginning. Those differences of isozyme expressive content caused the changes of activity, which indicated SDS-PAGE electrophoresis and PAGE electrophoresis were reliable in the research of sijihua longan’s flower bud differentiation, but the related question was still worth researching deeply.更多还原